Mkn 45
Manufactured by iCell Bioscience
Sourced in China
The MKN-45 is a cell culture device used for the in vitro cultivation of human gastric adenocarcinoma cells. It provides a controlled environment for the growth and maintenance of these specialized cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Ges 1, by iCell Bioscience (3 mentions)
Sgc 7901, by iCell Bioscience (3 mentions)
Penicillin streptomycin sulfate, by Thermo Fisher Scientific (2 mentions)
Bgc 823, by iCell Bioscience (2 mentions)
Mgc 803, by iCell Bioscience (2 mentions)
Mg132, by Selleck Chemicals (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Sgc 7901, by Yuanye Bio-Technology (1 mentions)
Sgc 7901, by Thermo Fisher Scientific (1 mentions)
Mkn45, by Thermo Fisher Scientific (1 mentions)
Sybr green premix ex taqtm, by Takara Bio (1 mentions)
Ges 1, by Beyotime (1 mentions)
Hgc 27, by Beyotime (1 mentions)
Iscript cdna synthesis kit, by Bio-Rad (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Total rna extraction kit, by Takara Bio (1 mentions)
Cycloheximide (chx), by Selleck Chemicals (1 mentions)
6 protocols using mkn 45
1
Cultured Gastric Cell Lines: GES-1 and MKN-45
2
Gastric Cancer Cell Line Cultivation
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Human GC cell lines, including AGS, BGC-823, MGC-803, MKN45, SGC-7901, and human normal gastric epithelial cell line GES-1 were purchased from iCell Bioscience Inc. (Shanghai, China). AGS, BGC-823, and MGC-803 were cultured in DMEM medium, and MKN45, SGC-7901, and GES-1 were cultured in RPMI-1640 medium. All the mediums were purchased from Gibco (Carlsbad, CA, USA) and added 10% fetal bovine serum (Gibco) and 1% penicillin-streptomycin sulfate (Gibco). MG132 was obtained from Selleckchem (Radnor, PA, USA) and 10 μM of MG132 was used to treat cells.
3
Establishing 5-Fu Resistant Gastric Cancer Cell Line
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Human GC tumor cell lines SGC-7901 and MKN-45 were purchased from iCell Bioscience (Shanghai, China). The 5-Fu-sensitive gastric cancer line, SGC-7901, was cultured in RPMI 1640 medium (Gibco, Gaithersburg, MD, USA) containing 10% fetal bovine serum (Gibco, Gaithersburg, MD, USA) and 1% penicillin-streptomycin (Gibco, Gaithersburg, MD, USA) at 37 °C in a 5% CO2 incubator. The cells were digested with 0.25% trypsin and routinely passaged when the cells grew to 70–80% confluency. The stepwise addition method was used, starting from addition of 10 nmol/L 5-Fu (Yuanye Bio-Technology, Shanghai, China) until the cells can grew stably in culture medium containing 10 μM 5-Fu; the constructed drug-resistant cell line was named SGC-7901-5-Fu. The drugs were withdrawn 2 weeks before the experiment and routinely cultivated for future use.
4
Cultivation of MKN-45 Gastric Cancer Cells
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The human gastric cancer cell line MKN-45 was purchased from iCell Bioscience Inc, which was authenticated by short tandem repeats (STR) profiling and confirmed to be mycoplasma-free. MKN-45 cells were cultured in the RPMI-1640 medium (Invitrogen, CA, USA) supplemented with 10% fetal bovine serum (Gibco, Australia) and penicillin/streptomycin (Gibco, MA, USA) at 37°C in a 5% CO2 water-saturated atmosphere. 3.
5
Quantification of Gastric Cancer CircRNA Levels
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Human gastric epithelial cell line GES-1 and GC cell lines HGC-27 were obtained from Beyotime (ShangHai, China), GC cell lines AGS were derived from American Tissue Culture Collection (ATCC, Rockville, MD, USA) while MKN-45 were derived from iCell Bioscience Inc, (ShangHai, China). All cells were incubated in RPMI-1640 (GIBCO, Invitrogen Corporation, NY, USA), supplemented with 10% fetal bovine serum (FBS) (Hyclone, Logan, UT, USA). All cell lines were grown at 37˚C in 5% CO 2 .
Total RNA of cells were extracted by Total RNA extraction kit (Takara, Japan) and reverse transcribed into cDNA using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). CircRNA expression level was measured by qRT-PCR using SYBR Green Premix Ex TaqTM (Takara, Japan) on ABI 7500 real-time uorescence quantitative PCR instrument (Applied Biosystems Inc, USA). Primers for circRNAs and GAPDH were as follows: hsa_circ_0001998: F-CCTGGCGTTGGATTATGCTC and R-AAGCAGCAACCGGAGAGATT; hsa_circ_0002504: F-TTTCGTTTTGATGGCTGGCT and R-TCATCTTTGATGCTGTTGTGCT; GAPDH: F-GCACCGTCAAGGCTGAGAAC and R-TGGTGAAGACGCCAGTGGA.
Total RNA of cells were extracted by Total RNA extraction kit (Takara, Japan) and reverse transcribed into cDNA using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). CircRNA expression level was measured by qRT-PCR using SYBR Green Premix Ex TaqTM (Takara, Japan) on ABI 7500 real-time uorescence quantitative PCR instrument (Applied Biosystems Inc, USA). Primers for circRNAs and GAPDH were as follows: hsa_circ_0001998: F-CCTGGCGTTGGATTATGCTC and R-AAGCAGCAACCGGAGAGATT; hsa_circ_0002504: F-TTTCGTTTTGATGGCTGGCT and R-TCATCTTTGATGCTGTTGTGCT; GAPDH: F-GCACCGTCAAGGCTGAGAAC and R-TGGTGAAGACGCCAGTGGA.
6
Gastric Cancer Cell Line Culture
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Human GC cell lines, including AGS, BGC-823, MGC-803, MKN45, SGC-7901, and human normal gastric epithelial cell line GES-1 were purchased from iCell Bioscience Inc. (Shanghai, China). AGS, BGC-823, and MGC-803 were cultured in DMEM medium, and MKN45, SGC-7901, and GES-1 were cultured in RPMI-1640 medium. All the mediums were purchased from Gibco (Carlsbad, CA, USA) and 10% fetal bovine serum (Gibco) and 1% penicillin-streptomycin sulfate (Gibco) were added. 10 μM of MG132 (Selleckchem, Radnor, PA, USA), a proteasome inhibitor, or 100 μg/ml of cycloheximide (Selleckchem), a protein synthesis inhibitor were used to treat cells.
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