Q plex array

Manufactured by Quansys Biosciences

The Q-Plex array is a multiplex assay platform developed by Quansys Biosciences. It allows for the simultaneous quantification of multiple analytes in a single sample.

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Lab products found in correlation

Hemolysis buffer, by Immuno-Biological Laboratories (1 mentions) Hepes buffer, by Merck Group (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Merck Group (1 mentions) Elisa kit, by InvivoGen (1 mentions) Q view, by Quansys Biosciences (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions)

11 protocols using q plex array

Cytokine Induction Assay in Mice

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Cardiac
blood was collected
from BALB/c mice 3 and 24 h after tail vein injection of 100 μL
containing PBS, PB84 (4 mg/kg) in PBS, or immunogenic
lipopolysaccharide (LPS 0.05 mg/mL). Serum was stored at −80
°C before shipment for analysis of induction of cytokines via
a custom murine Q-Plex array (Quansys Biosciences). The custom array
included IFN-α, IFN-β, IFN-γ, IL-1b, IL-6, IL-12,
TNF, CXCL1, and CXCL2.

Wamhoff E.C., Knappe G.A., Burds A.A., Du R.R., Neun B.W., Difilippantonio S., Sanders C., Edmondson E.F., Matta J.L., Dobrovolskaia M.A, & Bathe M. (2023). Evaluation of Nonmodified Wireframe DNA Origami for Acute Toxicity and Biodistribution in Mice. ACS Applied Bio Materials, 6(5), 1960-1969.

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Evaluating Splenocyte Proliferation and Cytokine Release

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Splenocytes were collected from mouse spleen 21 days after EAE induction. Spleen were dissected and triturated by using 1-ml syringe and 100-µm nylon cell strainer in RPMI1640 (Sigma), and then cells were treated with hemolysis buffer (Immuno-Biological Laboratories). After centrifugation, cells were suspended in RPMI1640 containing 10 mM HEPES buffer (Sigma), 10% FBS, 50 μM 2-mercaptoethanol, and penicillin-streptomycin (Gibco). Cells were cells were plated at density of 1 × 10⁴ cells/well into 96-well plates and were maintained at 37°C with 5% CO₂. To evaluate cell proliferation, cells were cultured with 20 μg/mL MOG_35-55 peptide for 72 hr. Cell proliferation was assessed using the Cell Proliferation ELISA and BrdU (colorimetric) kit (Sigma). BrdU solution was added into the culture 24 hr before the end of culturing. To evaluate the cytokine release, cells were cultured with 20 μg/mL MOG_35-55 peptide for 72 hr. The supernatants of the culture were collected and were used for the analysis of cytokine level by Q–Plex array (Quansys Biosciences).

Hamaguchi M., Muramatsu R., Fujimura H., Mochizuki H., Kataoka H, & Yamashita T. (2019). Circulating transforming growth factor-β1 facilitates remyelination in the adult central nervous system. eLife, 8, e41869.

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Multiplex Cytokine/Chemokine Profiling

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A sample (200 μl) from each lung homogenate was tested for
cytokines and chemokines using a chemiluminescent assay according to the
manufacturer’s instructions (Quansys Biosciences Q-Plex™ Array,
Logan, UT). The Quansys multiplex ELISA is a quantitative test in which 16
distinct capture antibodies are applied to each well of a 96-well plate in a
defined array. Each sample supernatant was tested at 2 dilutions for the
following: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10,
IL-12p70, IL-17, MCP-1, IFN-γ, TNFα, MIP-1α, GM-CSF, and
RANTES. Cytokine and chemokine concentrations are reported as fold changes
compared to measurements from uninfected control mice (baseline). Definition of
abbreviations are: IL - interleukin; MCP - monocyte chemoattractant protein; IFN
- interferon; TNF - tumor necrosis factor, MIP – macrophage inflammatory
protein; GM-CSF - granulocyte/ macrophage colony stimulating factor; and RANTES
- regulated upon activation, normal T cell expressed and secreted.

Joseph Evans W., Hurst B.L., Peterson C.J., Van Wettere A.J., Day C.W., Smee D.F, & Bart Tarbet E. (2018). Development of a Respiratory Disease Model for Enterovirus D68 in 4-Week-Old Mice for Evaluation of Antiviral Therapies. Antiviral research, 162, 61-70.

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Multiplex Cytokine Quantification in Lung

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Each sample of lung homogenate was tested for cytokines and chemokines using quantitative chemiluminescent ELISA-based assays according to the manufacturer’s instructions (Quansys Biosciences Q-Plex™ Array, Logan, UT). The Quansys multiplex ELISA is a quantitative test in which 16 distinct capture antibodies are applied to each well of a 96-well plate in a defined array.

Vogt M.R., Fu J., Kose N., Williamson L.E., Bombardi R., Setliff I., Georgiev I.S., Klose T., Rossmann M.G., Bochkov Y.A., Gern J.E., Kuhn R.J, & Crowe JE J.r. (2020). Human Antibodies Neutralize Enterovirus D68 and Protect Against Infection and Paralytic Disease. Science immunology, 5(49), eaba4902.

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Plasma Cytokine and Chemokine Profiles

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Enzyme-linked immunosorbent assay (ELISA) kits (InvivoGen) were used to measure IFN-α and IFN-β in the plasma following the manufacturer’s instructions. The plates were scanned with FLUOstar Omega microplate reader. Limits of detection: IFN-α, 7.8–500 pg/ml and IFN-β, 15.6–1000 pg/ml. A Q-plex array (Quansys Biosciences) was used to determine cytokine and chemokine levels in plasma. The plate was scanned with Odyssey Infrared Imaging System (LI-COR Biosciences) and analyzed with Q-View (Quansys Biosciences). Limits of detection: TNF, 4.12–3000 pg/ml; IL-1β, 19.2–14000 pg/ml; IL-5, 6.86–5000 pg/ml; IL-6, 6.86–5000 pg/ml; IFN-γ, 10.97–8000 pg/ml; CCL1, 5.6–4000 pg/ml; CCL2, 4.12–3000 pg/ml; CCL22, 5.6–4000 pg/ml.

Jung S.R., Ashhurst T.M., West P.K., Viengkhou B., King N.J., Campbell I.L, & Hofer M.J. (2020). Contribution of STAT1 to innate and adaptive immunity during type I interferon-mediated lethal virus infection. PLoS Pathogens, 16(4), e1008525.

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Cytokine Profile Analysis in Plasma

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Plasma samples were tested by Quansys Biosciences (Logan, Utah), using QPlex Array kits for human cytokines for the detection of IL7, IL17, IL10, transforming growth factor beta, interferon, tumor necrosis factor alpha (TNF), TNF receptor 1, and IL12p40.

Perez L., Fernandez H., Horna P., Riches M., Locke F., Field T., Powers J., Sahakian E., Villagra A., Mishra A., Betts B., Kharfan-Dabaja M., Beato F., Ochoa-Bayona L., Pidala J, & Anasetti C. (2018). Phase I trial of histone deacetylase inhibitor panobinostat in addition to glucocorticoids for primary therapy of acute graft-versus-host disease. Bone marrow transplantation, 53(11), 1434-1444.

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Multiplex ELISA Cytokine Profiling

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A sample (20 μl) from each lung homogenate was tested for cytokines and chemokines using an ELISA-based assay according to the manufacturer’s instructions (Quansys Biosciences Q-Plex™ Array, Logan, UT). The Quansys multiplex ELISA is a quantitative test in which 16 distinct capture antibodies have been applied to each well of a 96-well plate in a defined array. Each sample supernatant was tested at 2 dilutions for the following: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17, MCP-1, IFN-γ, TNFα, MIP-1α, GM-CSF, and RANTES. Cytokine and chemokine titers of mice infected with EV-D68 are reported in pg/ml of homogenized lung tissue. Definition of abbreviations are: IL -interleukin; MCP - monocyte chemoattractant protein; IFN - interferon; TNF -tumor necrosis factor, MIP – macrophage inflammatory Protein; GM-CSF -granulocyte/macrophage colony stimulating factor; and RANTES – regulated upon activation, normal T cell expressed and secreted.

Hurst B.L., Evans W.J., Smee D.F., Van Wettere A.J, & Tarbet E.B. (2018). Evaluation of antiviral therapies in respiratory and neurological disease models of Enterovirus D68 infection in mice.. Virology, 526, 146-154.

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Cytokine Induction in BALB/c Mice

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Cardiac blood was collected from BALB/c mice 3 hr and 24 hr after tail vein injection of 100 μL containing PBS, PB84 (4mg/kg) in PBS, or immunogenic lipopolysaccharide (LPS 0.05mg/mL). Serum was stored at −80°C before shipment for analysis of induction of cytokines via a custom murine Q-Plex array (Quansys Biosciences). The custom array included IFN-α, IFN-β, IFN-γ, IL-1b, IL-6, IL-12, TNF, CXCL1 and CXCL2.

Wamhoff E.C., Knappe G.A., Burds A.A., Du R.R., Neun B.W., Difilippantonio S., Sanders C., Edmondson E.F., Matta J.L., Dobrovolskaia M.A, & Bathe M. (2023). Evaluation of non-modified wireframe DNA origami for acute toxicity and biodistribution in mice. bioRxiv.

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Lung Virus Titer and Cytokine Profiling

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Six days after virus challenge, the bronchioalveolar lavage (BAL) procedure was performed immediately after blood collection and was completed within 5–10 min postmortem. A volume of 0.75 mL of PBS was slowly delivered into the lung through the tracheal tube. Immediately after delivery, the fluid was slowly withdrawn by gentle suction and the samples were stored at −80°C. The procedure was repeated a total of three times and lavage fluids from each mouse were pooled. To determine lung virus titers, BAL samples were centrifuged at 2,000 g for 5 min. Varying 10-fold dilutions of BAL supernatants were assayed in triplicate for infectious virus in MDCK cells, with virus titers calculated as described previously (67 (link)). For determination of lung cytokine levels, a sample (200 μL) from each lung lavage was tested for MCP-1 and IL-6 using a chemiluminescent multiplex ELISA-based assay according to the manufacturer's instructions (Quansys Biosciences Q-Plex™ Array, Logan, UT).

Sato-Kaneko F., Yao S., Lao F.S., Shpigelman J., Messer K., Pu M., Shukla N.M., Cottam H.B., Chan M., Chu P.J., Burkhart D., Schoener R., Matsutani T., Carson D.A., Corr M, & Hayashi T. (2020). A Novel Synthetic Dual Agonistic Liposomal TLR4/7 Adjuvant Promotes Broad Immune Responses in an Influenza Vaccine With Minimal Reactogenicity. Frontiers in Immunology, 11, 1207.

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Cytokine Induction in BALB/c Mice

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Tanaka M., Kato K., Hakusui H., Murakami Y., Sato K., Ito Y, & Kawamoto K. (2000). Pharmacokinetics and Safety of Ascending Single Doses of DZ-2640, a New Oral Carbapenem Antibiotic, Administered to Healthy Japanese Subjects. Antimicrobial Agents and Chemotherapy, 44(3), 578-582.

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