Bisbenzimide h33342 trihydrochloride hoechst 33342

Manufactured by Beyotime

Sourced in China

Bisbenzimide H33342 trihydrochloride (Hoechst 33342) is a fluorescent dye used in laboratory applications. It binds to DNA and emits a blue-violet fluorescence upon excitation, allowing for the detection and visualization of cells and nuclei.

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Lab products found in correlation

High glucose dulbecco s modified eagle s medium dmem, by Cytiva (1 mentions) Propidium iodide, by Beyotime (1 mentions) Doxorubicin hydrochloride dox hcl, by Meilun (1 mentions)

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Hoechst 33342 (660 citations) Hoechst (153 citations)

2 protocols using bisbenzimide h33342 trihydrochloride hoechst 33342

Dandelion-Based Antioxidant Potential in PC12 Cells

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Dandelion was harvested in May 2022 at Tai’an, Shandong, China. These dandelions were identified as the dry whole plant of Taraxacum mongolicum by Professor Xiao Wang of Shandong University of Traditional Chinese Medicine. High glucose Dulbecco’s Modified Eagle’s medium (DMEM) was obtained from Hyclone Laboratories (Logan, UT, USA). 2′,7′-dichlorofluorescin diacetate (DCFH-DA), MitoTracker Red fluorescent probe, and Bisbenzimide H33342 trihydrochloride (Hoechst 33342) were supplied by Beyotime Biotechnology Co., Ltd. (Shanghai, China). Undecanoic acid, 19-decanoic acid were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). L-d₅-phenylalanine was bought from Bai J&K Scientific Co., Ltd. (Beijing, China). Rat Adrenal Pheochromocytoma Cells (PC12 cells) were bought from Procell Life Science & Technology Co., Ltd. (Wuhan, China). Methanol and other chemical reagents were of chromatographic grade.

Chen Y., Fei S., Yu X, & Tan M. (2023). Dandelion (Taraxacum mongolicum) Extract Alleviated H2O2-Induced Oxidative Damage: The Underlying Mechanism Revealed by Metabolomics and Lipidomics. Foods, 12(17), 3314.

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Synthesis of Drug Delivery Nanoparticles

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N-Carbobenzyloxy-N-(2,4-dinitrophenyl)-2-histidine (N-CBZ-N-DNP-2-histidine) and β-benzyl-L-aspartate were purchased from GL Biochem (Shanghai, China) Ltd. Triphosgene was purchased from TCI (Japan). BisBenzimide H 33342 trihydrochloride (Hoechst 33342) and propidium iodide (PI) were purchased from Beyotime Biotechnology Ltd. (Shanghai, China). Poly(ethylene glycol 2000) methyl ether amine (CH₃O-PEG2000-NH₂), Lysotracker^TM Green and (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) were gotten from Sigma-Aldrich Shanghai Trading Co. Ltd. (Shanghai, China). Other reagents were bought from Alfa Aesar (China) Chemical Co. Ltd. (Shanghai, China). Doxorubicin hydrochloride (DOX·HCl) and Combretastatin A4 (CA4) were purchased from Dalian Meilun Biotech Co. Ltd. (Dalian, China). Doxorubicin hydrochloride was deprotonated with triethylamine in dimethylsulphoxide (DMSO) to obtain the hydrophobic DOX.

Dong Y., Yang J., Liu H., Wang T., Tang S., Zhang J, & Zhang X. (2015). Site-Specific Drug-Releasing Polypeptide Nanocarriers Based on Dual-pH Response for Enhanced Therapeutic Efficacy against Drug-Resistant Tumors. Theranostics, 5(8), 890-904.

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