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Gapdh sc25788

Manufactured by Santa Cruz Biotechnology

GAPDH (sc-25788) is a highly specific antibody that recognizes the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein. GAPDH is a key enzyme involved in glycolysis, catalyzing the conversion of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate. This antibody can be used to detect and quantify GAPDH expression in a variety of sample types.

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2 protocols using gapdh sc25788

1

Antibody Panel for Adipose Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The protease inhibitor cocktail (cOmplete Mini) and a phosphatase inhibitor cocktail (PhosSTOP) were obtained from Roche Diagnostics. The following antibodies were obtained from Cell Signaling Technology: Cox4 (4844), PPARγ (2435), C/EBPα (8178), p-Ser239 VASP (3114), total VASP (3112), p-Ser473 AKT (4060), p-Thr308 AKT (9275), total AKT (9272), p-Ser563 HSL (4139), total HSL (4107), ATGL (2439), ADIPOQ (2789), GLUT4 (2213), ACC (3676), FASN (3180), and β-actin (4967). Other antibodies used include NPRC (NBP1–31365, Novus Biologicals), NPRA (NBP1–31333, Novus Biologicals), NDUFS4 (PA5–21677, Pierce Biotechnology), UCP1 (ab23841, Abcam), and GAPDH (sc25788, Santa Cruz Biotechnology) and CYTOC (sc13156, Santa Cruz Biotechnology). Secondary antibodies against rabbit immunoglobulin G (IgG) (A3687) and mouse IgG (A3562), both conjugated with alkaline phosphatase, were from Sigma-Aldrich.
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2

Antibody Panel for Adipose Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
The protease inhibitor cocktail (cOmplete Mini) and a phosphatase inhibitor cocktail (PhosSTOP) were obtained from Roche Diagnostics. The following antibodies were obtained from Cell Signaling Technology: Cox4 (4844), PPARγ (2435), C/EBPα (8178), p-Ser239 VASP (3114), total VASP (3112), p-Ser473 AKT (4060), p-Thr308 AKT (9275), total AKT (9272), p-Ser563 HSL (4139), total HSL (4107), ATGL (2439), ADIPOQ (2789), GLUT4 (2213), ACC (3676), FASN (3180), and β-actin (4967). Other antibodies used include NPRC (NBP1–31365, Novus Biologicals), NPRA (NBP1–31333, Novus Biologicals), NDUFS4 (PA5–21677, Pierce Biotechnology), UCP1 (ab23841, Abcam), and GAPDH (sc25788, Santa Cruz Biotechnology) and CYTOC (sc13156, Santa Cruz Biotechnology). Secondary antibodies against rabbit immunoglobulin G (IgG) (A3687) and mouse IgG (A3562), both conjugated with alkaline phosphatase, were from Sigma-Aldrich.
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