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Taurodeoxycholic acid tdca

Manufactured by Merck Group
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Taurodeoxycholic acid (TDCA) is a synthetic bile acid used in various laboratory applications. It is a white crystalline powder that is soluble in water and organic solvents. TDCA is commonly used as a surfactant and emulsifier in biological and biochemical research.

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Lab products found in correlation

Calcium chloride, by Merck Group (1 mentions) Taurocholic acid tca, by Merck Group (1 mentions) Glycocholic acid gca, by Merck Group (1 mentions) L valine, by Merck Group (1 mentions) Glycochenodeoxycholic acid gcdca, by Merck Group (1 mentions) Acetonitrile, by Avantor (1 mentions) Formic acid, by Avantor (1 mentions) Hplc grade methanol, by Avantor (1 mentions)

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Taurodeoxycholic acid (64 citations)

4 protocols using taurodeoxycholic acid tdca

1

Bile Salt Hydrolase Activity in Bacteria

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The bacteria were streaked on modified MRS agar and added extra 0.5% (w/v) sodium salt of Tauro deoxycholic acid (TDCA) (Sigma-Aldrich, St. Louis, MO, USA) and 0.37 g/L of calcium chloride (Sigma-Aldrich, USA). Plates were incubated at 37 °C for 72 h. A halo zone around colonies was observed (indicating bile salt hydrolase activity existed).
Xiao J., Chen C., Fu Z., Wang S, & Luo F. (2024). Assessment of the Safety and Probiotic Properties of Enterococcus faecium B13 Isolated from Fermented Chili. Microorganisms, 12(5), 994.
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2

Bile Salt Hydrolase Activity Assay

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The BSH activity of probiotic strains was based on the method described by Ayama et al. [11 ], Kumar et al. [23 (link)], and Pereira et al. [24 (link)]. The suspensions of probiotics were spotted on MRS agar plates supplemented with 0.5% w/v conjugated bile salt (glycocholic acid (GCA), taurocholic acid (TCA), and taurodeoxycholic acid (TDCA) from Fluka, Sigma-Aldrich, St. Louis, MO, USA) and 0.37 g/L calcium chloride. The MRS agar plate without supplementation with bile salts was used as a control. The plates were anaerobically incubated at 37 °C for 24, 48, and 72 h. BSH activity was determined through precipitation zones around the colonies.
Kaewarsar E., Chaiyasut C., Lailerd N., Makhamrueang N., Peerajan S, & Sirilun S. (2023). Effects of Synbiotic Lacticaseibacillus paracasei, Bifidobacterium breve, and Prebiotics on the Growth Stimulation of Beneficial Gut Microbiota. Foods, 12(20), 3847.
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3

Dietary Intervention and Metabolic Modulation

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DIO-C57BL/6 mice animals that were on a HFD at 6 weeks of age for a duration of 12 weeks were then switched to a normal chow diet (NCD) and consecutive body weight was assessed.
Taurodeoxycholic acid (TDCA) (50mg/kg) and L-valine (200mg/kg) (both from Sigma Aldrich, St. Louis, MO) were dissolved in water and simultaneously administered by intraperitoneal injection.
Quante M., Iske J., Uehara H., Minami K., Nian Y., Maenosono R., Matsunaga T., Liu Y., Azuma H., Perkins D., Alegre M.L., Zhou H., Elkhal A, & Tullius S.G. (2021). Taurodeoxycholic Acid and Valine Reverse Obesity-Associated Augmented Alloimmune Responses and Prolong Allograft Survival. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 22(2), 402-413.
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4

Bile Acid Metabolites Analysis Protocol

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CA was purchased from Alfa Aesar (Ward Hill, MA), taurocholic acid (TCA) from Acros Organics (Pittsburgh, PA) and glycocholic acid (GCA) from Pfaltz & Bauer (West Chester, PA). CDCA, glycochenodeoxycholic acid (GCDCA), taurochenoxycholic acid (TCDCA), deoxycholic acid (DCA), glycodeoxycholic acid (GDCA), taurodeoxycholic acid (TDCA) and deoxy-2,2,4,4-d4 acid (DCA-d4) were purchased from Sigma-Aldrich (St. Louis, MO). Cholic-2,2,4,4-d4 acid (CA-d4), glycocholic-2,2,4,5-d4 acid (GCA-d4), chenodeoxycholic-2,2,4,4-d4 acid (CDCA-d4) and glycochenodeoxycholic-2,2,4,4-d4 acid (-GCDCA-d4) were purchased from C/D/N ISOTOPES Inc. (Quebec, Canada). Taurocholic 2,2,3,4,4-d5 acid (TCA- d5) and taurochenodeoxycholic-2,2,3,4,4-d5 acid (TCDCA-d5) were purchased from Toronto Research Chemicals Inc. (Ontario, Canada). HPLC grade methanol, acetonitrile, water and formic acid were purchased from Honeywell Burdick & Jackson (Muskegon, MI). Charcoal stripped serum was purchased from Bioreclamation (Westbury, NY).
Luo L., Aubrecht J., Li D., Warner R.L., Johnson K.J., Kenny J, & Colangelo J.L. (2018). Assessment of serum bile acid profiles as biomarkers of liver injury and liver disease in humans. PLoS ONE, 13(3), e0193824.
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