Log In Sign up
The largest database of trusted experimental protocols

Hexim1

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

HEXIM1 is a protein that functions as a negative regulator of transcription elongation. It interacts with the positive transcription elongation factor b (P-TEFb) complex, inhibiting its kinase activity and thereby suppressing the transcription of target genes.

Automatically generated - may contain errors

Lab products found in correlation

C myc, by Santa Cruz Biotechnology (3 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) Odyssey clx imager, by LI COR (2 mentions) Ripa buffer, by Boston BioProducts (2 mentions) Halt protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Caspase glo 3 7 assay, by Promega (1 mentions) Biocoat matrigel invasion chamber, by Corning (1 mentions) Rneasy plus kit, by Qiagen (1 mentions) Celltiter blue cell viability assay, by Promega (1 mentions) P3xflag cmv 7, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Carboplatin, by Selleck Chemicals (1 mentions) Paclitaxel, by Selleck Chemicals (1 mentions) Nup98, by Cell Signaling Technology (1 mentions) Rnapii, by Cell Signaling Technology (1 mentions) Abbv744, by Selleck Chemicals (1 mentions) Migration chamber, by Ibidi (1 mentions) Pcas9 bb 2a puro px459 v2, by Addgene (1 mentions) Caspase 8, by Enzo Life Sciences (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions)

4 protocols using hexim1

1

Comprehensive Protein Analysis in Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies and sources: CDK9 (1.1000), pCDK9 (Thr187) (1.1000), RNAPII (1.1000), phospho-RNAPII(Ser2) (1.1000), PARP (1.1000), and NUP98 (1.1000) (Cell Signaling Technology); HEXIM1 (1.1000), NELF-a (1.1000), LARP-7 (1.1000), c-Myc (1.1000), SPT5 (1.1000), and GAPDH (1.5000) (Santa Cruz Biotechnology, Dallas, TX, USA); BRD4 (1.1000) (Abcam, Waltham, MA, USA); Caspase-8 (1.1000) (Enzo Life Sciences, Farmingdale, NY, USA); β-Actin (1.10000), (Sigma-Aldrich, St. Louis, MO, USA); Cyclin T (1.1000) (Bethyl, Montgomery, TX, USA). Reagents and sources: CellTiter-Blue Cell Viability assay and Caspase-Glo 3/7 assays (Promega, Madison, WI, USA); AnnexinV and 7AAD (BD); BAY1251152, BI894999, ABBV744, Paclitaxel and Carboplatin (Selleckchem, Houston, TX, USA); BioCoat Matrigel invasion chamber (Corning, Corning, NY, USA); Migration chamber (Ibidi, Gräfelfing, Germany); RNeasy Plus kit (Qiagen, Venlo, The Netherlands). The following vectors were used: pCas9(BB)-2A-Puro (PX459) V2.0 (62988, Addgene, Watertown, MA, USA); p3xFlag-CMV-7.1 (E7533, Sigma, Ronkonkoma, NY, USA).
Gasimli K., Raab M., Mandal R., Krämer A., Peña-Llopis S., Tahmasbi Rad M., Becker S., Strebhardt K, & Sanhaji M. (2023). Synergistic Sensitization of High-Grade Serous Ovarian Cancer Cells Lacking Caspase-8 Expression to Chemotherapeutics Using Combinations of Small-Molecule BRD4 and CDK9 Inhibitors. Cancers, 16(1), 107.
+ Open protocol
+ Expand
2

Quantitative Immunohistochemistry of Tumor Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
OpalTM 7-Color Fluorescent Immunohistochemistry (IHC) Kits were used according to the manufacturer’s instructions. The following antibodies were used: αSMA (Sigma; F3777), Col9 SANTA CRUZ; sc-376969), Pdgfra (Cell Signaling; #3174), Rgs5 (Biozol; bs-2794R), Top2a (Biozol; orb379272), p65 (Cell Signaling; #8242), Foxo1 (Cell Signaling; #2880), Sirt1 (Upstate; 07-131), Hexim1 (Cell Signaling; #12604), and Otx1 (Abcam; ab25985). Stained tumor and mammary gland sections were scanned using Vectra® 3 automated quantitative pathology imaging system and analyzed using InForm software V2.3. Expression of markers in cells was quantified using the Scoring algorithm of the InForm software, either 4 bin (Hexim1) or double positivity (all others markers) scoring.
Elwakeel E., Brüggemann M., Fink A.F., Schulz M.H., Schmid T., Savai R., Brüne B., Zarnack K, & Weigert A. (2019). Phenotypic Plasticity of Fibroblasts during Mammary Carcinoma Development. International Journal of Molecular Sciences, 20(18), 4438.
+ Open protocol
+ Expand
3

Western Blot Analysis of BRD Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed with RIPA buffer (Boston BioProducts) supplemented with halt protease inhibitor cocktail (Thermo) and 0.1% benzonase (Novagen) on ice for 20 minutes. The lysates were spun at 20,000xg for 15 minutes at 4°C. A BCA assay (Pierce) was used to quantify protein concentration. The following antibodies were used in this study: BRD4 (Bethyl labs, A301–985A, 1:5000 dilution), BRD3 (abcam, ab56342, 1:1000), BRD2 (Bethyl labs, A302–582A, 1:1000), c-MYC (Santa Cruz, sc-764, 1:1000), actin (Santa Cruz, sc-8432, 1:1000), HEXIM1 (Cell Signaling, 9064S, 1:1000), PARP (Cell Signaling, 9542S, 1:1000) and cleaved caspase 3 (Cell Signaling, 9579S, 1:1000). Blots were imaged after incubating with fluorescence-labeled secondary antibodies anti-mouse-680 (LI-COR, 926–68070, 1:7000) or anti-rabbit-800 (LI-COR, 926–32211, 1:7000) on the OdysseyCLxImager (LI-COR).
Tanaka M., Roberts J.M., Seo H.S., Souza A., Paulk J., Scott T.G., DeAngelo S.L., Dhe-Paganon S, & Bradner J.E. (2016). Design and Characterization of Bivalent BET Inhibitors. Nature chemical biology, 12(12), 1089-1096.
+ Open protocol
+ Expand
4

Western Blot Analysis of BRD Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed with RIPA buffer (Boston BioProducts) supplemented with halt protease inhibitor cocktail (Thermo) and 0.1% benzonase (Novagen) on ice for 20 minutes. The lysates were spun at 20,000xg for 15 minutes at 4°C. A BCA assay (Pierce) was used to quantify protein concentration. The following antibodies were used in this study: BRD4 (Bethyl labs, A301–985A, 1:5000 dilution), BRD3 (abcam, ab56342, 1:1000), BRD2 (Bethyl labs, A302–582A, 1:1000), c-MYC (Santa Cruz, sc-764, 1:1000), actin (Santa Cruz, sc-8432, 1:1000), HEXIM1 (Cell Signaling, 9064S, 1:1000), PARP (Cell Signaling, 9542S, 1:1000) and cleaved caspase 3 (Cell Signaling, 9579S, 1:1000). Blots were imaged after incubating with fluorescence-labeled secondary antibodies anti-mouse-680 (LI-COR, 926–68070, 1:7000) or anti-rabbit-800 (LI-COR, 926–32211, 1:7000) on the OdysseyCLxImager (LI-COR).
Tanaka M., Roberts J.M., Seo H.S., Souza A., Paulk J., Scott T.G., DeAngelo S.L., Dhe-Paganon S, & Bradner J.E. (2016). Design and Characterization of Bivalent BET Inhibitors. Nature chemical biology, 12(12), 1089-1096.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!