The following agents were used: ATP (Sigma Aldrich Co., St. Louis, MO; MP Biomedicals, Solon, OH, USA, Calbiochem Co., La Jolla, CA, and Roche Diagnostic Co., Indianapolis, IN), ADP (Sigma), AMP (Sigma), adenosine (Sigma), benzoylbenzoyl ATP (Sigma), oxidized ATP (Sigma), suramin (Wako, Tokyo, Japan), MIA (methyl isobutyl amiloride, Wako), DIDS (4,4′- Diisothiocyanatostilbene-2,2′-disulfonic acid, Sigma), EDTA (Dojindo, Tokyo, Japan), EGTA (Dojindo), dipyridyl (Sigma), CAS (Chrome Azurol S, Sigma), E. coli S17-1 and pK18mobSacB (kindly provided by Dr. F. Taguchi, Okayama University), Instagene matrix (Bio-Rad, Hercules, CA), KOD-Plus (Toyobo, Osaka, Japan), Wizard SV Gel and PCR cleanup system (Promeg, Madison, WI), Ligation High (Toyobo), Protein Assay Rapid Kit (Wako), [14C] isoleucine (Moravek Biochemicals, Inc, Brea, CA), [14C]uracil (Moravek Biochemicals, Inc.), α-defensin-1 (Peptide institute, Inc, Osaka, Japan.), cathepsin G (Sigma), vancomycin (Wako), clarithromycin (Taisho-Toyama Pharmaceutical Co., Tokyo), rifampin (Daiichi Sankyo Co., Tokyo), and ethambutol (Sigma), gatifloxacin (Wako), FLUOS (Sigma), LB medium (Invitrogen, San Diego, CA), M9 medium (prepared by our laboratory), Heart infusion agar (Eiken Chemical Co., Tokyo, Japan), Middlebrook 7H9 medium (Becton Dickinson, Cockeysville, MD), and Middlebrook 7H11 medium (Becton Dickinson).
Oxidized atp
Manufactured by Merck Group
Sourced in United States
Oxidized ATP is a laboratory reagent used in biochemical and biophysical research. It is the oxidized form of adenosine triphosphate (ATP), a fundamental energy-carrying molecule in living cells. Oxidized ATP is commonly used as a molecular probe to study cellular processes and signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Adenosine triphosphate (atp), by Merck Group (2 mentions)
Adenosine, by Merck Group (1 mentions)
Dipyridyl, by Merck Group (1 mentions)
Vancomycin, by Fujifilm (1 mentions)
Ethambutol, by Merck Group (1 mentions)
Protein assay rapid kit, by Fujifilm (1 mentions)
Middlebrook 7h9 medium, by BD (1 mentions)
Ethylene glycol tetraacetic acid (egta), by Dojindo Laboratories (1 mentions)
Middlebrook 7h11 medium, by BD (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Dojindo Laboratories (1 mentions)
Benzoylbenzoyl atp, by Merck Group (1 mentions)
Lb medium, by Thermo Fisher Scientific (1 mentions)
Cathepsin g, by Merck Group (1 mentions)
Ligation high, by Toyobo (1 mentions)
Adenosine diphosphate (adp), by Merck Group (1 mentions)
Instagene matrix, by Bio-Rad (1 mentions)
Kod plus, by Toyobo (1 mentions)
Recombinant murine m csf, by Thermo Fisher Scientific (1 mentions)
Recombinant human m csf, by Thermo Fisher Scientific (1 mentions)
β actin antibody, by Cell Signaling Technology (1 mentions)
5 protocols using oxidized atp
1
Characterization of Bacterial Antimicrobial Mechanisms
2
Osteoclast Differentiation Pathway Modulators
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Human recombinant SAA, murine recombinant M-CSF, human recombinant M-CSF, and murine RANKL were purchased from Peprotech (Rocky Hill, NJ, USA). Pam3CSK4 was purchased from Invitrogen (San Diego, CA, USA). Lipopolysaccharide (LPS) and oxidized ATP were purchased from Sigma-Aldrich (St. Louis, MO, USA). WRWWWW (WRW4) was synthesized from AnyGen (Gwangju, Korea). Polymyxin B, pertussis toxin, SB203580 and U0126 were purchased from Calbiochem (San Diego, CA, USA). The APC-Anti-mouse CD115(c-fms) was purchased from eBioscience (San Diego, CA, USA). TAPI-1 was purchased from Enzo Life Sciences (Plymouth Meeting, PA, USA). The phospho-ERK, ERK, phospho-p38 mitogen-activated protein kinase (MAPK), p38 MAPK and β-actin antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).
3
Inhibition of ATP/NCAMP-1 Effects on ZFCC
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Inhibition of ATP/NCAMP-1 induced effects on ZFCC activity was done by pretreatment with KN-62 ((S)-5-Isoquinolinesulfonic acid 4-[2-[(5-isoquinolinylsulfonyl)methylamino]-3-oxo-3-(4-phenyl-1-piperazinyl)propyl]phenyl ester, 1-[N,O-bis(5-Isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine, Sigma, I2142), Comassie Brilliant Blue (CBB), or oxidized-ATP (oATP produced by the 2’- and 3’-hydroxyl moieties oxidized to aldehydes by periodate treatment) (Sigma). Before analysis of ligand binding, YO-PRO-1 or Fluo-3 were added and pore formation or Ca2+ influx was measured as described above.
4
Investigating Purinergic Signaling in Cellular Processes
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ATP (adenosine 5'-triphosphate), Brilliant Blue G (BBG), PI, Triton X-100, Oxidized ATP (OATP), HEPES, NaCl, KCl, MgCl2, CaCl2, Mefloquine (MFQ), Carbenoxolone (CBX) and RPMI 1640 were purchased from Sigma Chemical Co., St. Louis, MO (USA). Fetal bovine serum (FBS) was obtained from Gibco BRL (USA). AZ11645373 (AZ) was obtained from Tocris Bioscience (United Kingdom).
5
Wistar Rat Inflammation and Calcium Study
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Healthy male Wistar rats with weight of 200 ± 50 g were obtained from Institute of Health and Environmental Medicine, Academy of Military Medical Sciences (Tianjin, China, Certification Number: SCXK 2010-0002). Dulbecco's modified Eagle's medium (DMEM), RPMI1640 medium and fetal calf serum (FCS) were purchased from Gibco (USA) and HyClone (USA), respectively. Rhein was obtained from National Institute for the Control of Pharmaceutical and Biological Products (China). Fura-2/AM was from Biotium (USA). Rat IL-1β ELISA kits were purchased from NeoBioscience Technology Co.,Ltd. (China). RNAprep pure Cell/Bacteria Kit was from TIANGEN BIOTECH (China). Reverse Transcription System, GoTaq PCR Core system and Caspase-Glo assay kit were from Promega (USA). FluoSpheres carboxylate-modified, 1.0 μm, nile red was purchased from Molecular Probes (USA). The rest of reagents, including ATP, BzATP, oxidized ATP, BBG, LPS, EB, DHE, rhodamine 123, dimethylsulfoxide (DMSO) and MTT were purchased from Sigma (USA).
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