Mouse anti sirt1

Manufactured by Abcam

Sourced in United Kingdom, United States, Japan

Mouse anti-SIRT1 is a primary antibody that specifically binds to and detects the SIRT1 protein. SIRT1 is a nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylase that plays a role in various cellular processes.

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5 protocols using mouse anti sirt1

Mitochondrial Dynamics Protein Analysis

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Equal amounts of proteins were separated by 10% SDS-PAGE under reducing conditions and then transferred to PVDF membranes (Merck Millipore, Darmstadt, Germany). The membranes were blocked using 5% skimmed milk in TBS supplemented with 0.1% Tween-20 and then incubated with the following antibodies: mouse anti-SIRT1 (1:1000; Abcam, Cambridge, UK), mouse anti-Drp1 (1:1000; Abcam), mouse anti-Opa1 (1:1000; Proteintech, Chicago, USA), rabbit anti-Mfn1 (1:1000; Abcam), rabbit anti-Mfn2 (1:1000; Abcam), mouse anti-NDUFA9 (1:1000; Proteintech), mouse anti-ATP5A1 (1:1000; Proteintech), rabbit anti-AKT (1:500; Santa Cruz Biotech, Santa Cruz, USA), rabbit anti-pAKT (1:500; Santa Cruz Biotech), and rabbit anti-GAPDH (1:2000; Proteintech). The signal was visualised using the corresponding horseradish peroxidase-conjugated goat anti-mouse or goat anti-rabbit secondary antibodies (1:5000; Proteintech) and enhanced chemiluminescence-western blotting detection reagent (Bioscience Biotech, Beijing, China). Protein bands were quantified by densitometry using Quantity One software.

Xu J., Zhang Y., Yu Z., Guan Y., Lv Y., Zhang M., Zhang M., Chen L., lv X, & Guan F. (2022). Berberine mitigates hepatic insulin resistance by enhancing mitochondrial architecture via the SIRT1/Opa1 signalling pathway: Berberine mitigates hepatic insulin resistance via SIRT1/Opa1. Acta Biochimica et Biophysica Sinica, 54(10), 1464-1475.

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Immunohistochemical Analysis of SIRT1, TFAM, COXIV, and TOMM20

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Immunohistochemical staining was performed on 5-μm sections of paraformaldehyde- fixed, paraffin-embedded tumor samples, adjacent nontumoral liver tissue, normal liver tissue and mouse lung tissue. The details of the immunohistochemistry were described previously [28 (link)]. The slides were incubated with mouse anti-SIRT1 (Abcam), rabbit anti-TFAM (Abcam), rabbit anti-COXIV (Cell Signaling Technology, Danvers, MA, USA) and rabbit anti-TOMM20 (Abcam) primary antibodies. The mouse lung tissue sections (5-μm thick) were stained with hematoxylin and eosin (H&E) so that tumor nodules could be observed. The slides were evaluated under a light microscope (Leica, DM6000M).

Li Y., Xu S., Li J., Zheng L., Feng M., Wang X., Han K., Pi H., Li M., Huang X., You N., Tian Y., Zuo G., Li H., Zhao H., Deng P., Yu Z., Zhou Z, & Liang P. (2016). SIRT1 facilitates hepatocellular carcinoma metastasis by promoting PGC-1α-mediated mitochondrial biogenesis. Oncotarget, 7(20), 29255-29274.

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Neuroprotective Mechanism of TC-G 1008

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The expression levels of GPR39, SIRT1, PGC-1α and Nrf2 were measured at 0 h, 6 h, 12 h, 24 h, 48 h, 72 h and 7 days post-HIE by Western blot following the manufacturer’s recommendations [34 (link), 35 (link)]. To analyze whether GPR39 receptor and SIRT1/PGC-1α/Nrf2 pathway were involved in the neuroprotective effects of TC-G 1008, the expression levels of GPR39, SIRT1, PGC-1α and Nrf2, and pivotal inflammatory cytokines IL-6, IL-1β, TNF-α were assessed via Western blot. RIPA lysis buffer (Santa Cruz Biotechnology, USA) was used to obtain whole cell lysates. Primary antibodies used were rabbit anti-GPR39 (1:500, Bioss), mouse anti-SIRT1 (1:2000, Abcam), rabbit anti-PGC-1α (1:1000, Abcam), rabbit anti-Nrf2 (1:1000, Abcam), rabbit anti-interleukin (IL)-1β (1:1000, Abcam), rabbit anti-interleukin (IL)-6 (1:1000, Abcam), mouse anti-TNF-α(1:500, Abcam) and mouse anti-β-actin(1:3000, Santa Cruz). The next day, the anti-rabbit (or anti-mouse) secondary antibodies (1:3000, Santa Cruz Biotechnology, USA) were incubated at room temperature for 1–2 h. The gray values were quantified and analyzed by Image J software (NIH).

Xie S., Jiang X., Doycheva D.M., Shi H., Jin P., Gao L., Liu R., Xiao J., Hu X., Tang J., Zhang L, & Zhang J.H. (2021). Activation of GPR39 with TC-G 1008 attenuates neuroinflammation via SIRT1/PGC-1α/Nrf2 pathway post-neonatal hypoxic–ischemic injury in rats. Journal of Neuroinflammation, 18, 226.

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Antibody Characterization for HBV Research

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The following antibodies were used in the study: rabbit anti-TARDBP (MBL, Life Science), mouse anti-HBV core antibody HB91 (Advanced Life Science Institute Inc., Saitama, Japan), rabbit anti-NTCP/SLC10A1, mouse anti-SIRT1, rabbit anti-Hsp90, rabbit anti-NPM1, rabbit anti-hnRNPC1 + C2, and rabbit anti-hnRNPK (Abcam), goat anti-GAPDH, mouse anti-β-actin, and mouse anti-FLAG M2 (Sigma-Aldrich), rabbit anti-SFPQ, rabbit anti-PARP1, rabbit anti-PTBP1, and mouse anti-PUF60 (GeneTex). The above antibodies were used for western blotting experiments, whereas anti-TARDBP and anti-FLAG M2 were also used for the immunofluorescence assay. For immunoprecipitation of the core-associated HBV genomes, the mouse anti-HBV core monoclonal antibody 2A21 (Institute of Immunology, Tokyo, Japan) was used while the FLAG M2 antibody was used to precipitate FLAG-tagged TARDBP.

Makokha G.N., Abe-Chayama H., Chowdhury S., Hayes C.N., Tsuge M., Yoshima T., Ishida Y., Zhang Y., Uchida T., Tateno C., Akiyama R, & Chayama K. (2019). Regulation of the Hepatitis B virus replication and gene expression by the multi-functional protein TARDBP. Scientific Reports, 9, 8462.

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Western Blot for SIRT1, Caspase-1, p16

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Cell lysates were obtained using RIPA buffer (150 mM NaCl, 10 mM Tris, pH 7.2, 0.1% SDS, 1.0% Triton X-100, 5 mM EDTA, pH 8.0) with protease inhibitor Cocktail (Roche Applied Science, Indianapolis, IN, USA). Protein concentration for each sample was evaluated by Bradford assay. Proteins (30 μg) were analysed by SDS-PAGE, and then transferred to nitrocellulose membrane (Bio-Rad, Hercules, CA, USA). 5% skim milk was used to block the membrane that was then incubated overnight with the primary antibody.
Mouse anti-SIRT1 (Abcam), rabbit anti-Caspase-1 p10 (Santa Cruz Biotechnology), mouse anti-p16^ink4a (Santa Cruz) and, rabbit anti-α-tubulin (Cell Signaling), were used as primary antibodies.
Secondary horseradish peroxidase-conjugated anti-mouse and anti-rabbit antibodies were from horse and goat respectively (Vector laboratories, CA, USA). Protein bands were visualized using Clarity ECL chemiluminescence substrate (Bio-Rad) with Uvitec Imager (UVItec,Cambridge,UK) and then quantified using ImageJ software.
Caspase 1 activity was measured as the ratio between band intensity of the pro-Caspasi-1 and of the cleaved Caspasi-1. Each measure was normalized with α-tubulin.

Matacchione G., Gurău F., Silvestrini A., Tiboni M., Mancini L., Valli D., Rippo M.R., Recchioni R., Marcheselli F., Carnevali O., Procopio A.D., Casettari L, & Olivieri F. (2021). Anti-SASP and anti-inflammatory activity of resveratrol, curcumin and β-caryophyllene association on human endothelial and monocytic cells. Biogerontology, 22(3), 297-313.

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