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Anti pmlc2 s18t19

Manufactured by Cell Signaling Technology
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Anti-pMLC2 (S18T19) is a laboratory reagent produced by Cell Signaling Technology. It is an antibody that specifically recognizes phosphorylation of myosin light chain 2 at serine 18 and threonine 19 residues.

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Lab products found in correlation

Anti tubulin, by Cell Signaling Technology (2 mentions) Peroxidase conjugated anti mouse igg, by Cell Signaling Technology (2 mentions) Anti pmypt1 t696, by Merck Group (2 mentions)

2 protocols using anti pmlc2 s18t19

1

Immunoblot Analysis of Myosin Phosphorylation

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Cells were seeded onto CellStar tissue culture plates and grown to confluence in Ham’s F-12 media supplemented with L-glutamine and 10% FBS at 37°C in 5% CO2. Cells were starved in serum-free media for 72 hours at 37°C in 5% CO2, then treated with vehicle, Af extract, or purified Alp 1 diluted in serum-free medium for 24 hours. Lysates were prepared in RIPA buffer and immunoblotted using the following primary antibodies: anti-pMYPT1 (T696) (Millipore), anti-pMLC2 (S18T19) (Cell Signaling Technologies) and anti-tubulin (Cell Signaling Technologies The following secondary antibodies were used: donkey anti-mouse (Jackson ImmunoResearch Laboratories, peroxidase-conjugated anti-mouse IgG or donkey anti-rabbit (Cell Signaling Technologies). Immunoblots were visualized using standard chemiluminescence protocols.
Balenga N.A., Klichinsky M., Xie Z., Chan E.C., Zhao M., Jude J., Laviolette M., Panettieri RA J.r, & Druey K.M. (2015). A fungal protease allergen provokes airway hyperresponsiveness in asthma. Nature communications, 6, 6763.
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2

Immunoblot Analysis of Myosin Phosphorylation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded onto CellStar tissue culture plates and grown to confluence in Ham’s F-12 media supplemented with L-glutamine and 10% FBS at 37°C in 5% CO2. Cells were starved in serum-free media for 72 hours at 37°C in 5% CO2, then treated with vehicle, Af extract, or purified Alp 1 diluted in serum-free medium for 24 hours. Lysates were prepared in RIPA buffer and immunoblotted using the following primary antibodies: anti-pMYPT1 (T696) (Millipore), anti-pMLC2 (S18T19) (Cell Signaling Technologies) and anti-tubulin (Cell Signaling Technologies The following secondary antibodies were used: donkey anti-mouse (Jackson ImmunoResearch Laboratories, peroxidase-conjugated anti-mouse IgG or donkey anti-rabbit (Cell Signaling Technologies). Immunoblots were visualized using standard chemiluminescence protocols.
Balenga N.A., Klichinsky M., Xie Z., Chan E.C., Zhao M., Jude J., Laviolette M., Panettieri RA J.r, & Druey K.M. (2015). A fungal protease allergen provokes airway hyperresponsiveness in asthma. Nature communications, 6, 6763.
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