Ficoll density gradient centrifugation

Manufactured by STEMCELL

Sourced in Canada, France

Ficoll density gradient centrifugation is a laboratory technique used to separate and isolate cells or other biological particles based on their density. It involves the use of a Ficoll solution, a synthetic polymer, which forms a density gradient during centrifugation. This allows different cell types or particles to be separated and collected based on their specific density characteristics.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd3 cd28 beads, by Thermo Fisher Scientific (2 mentions) Easysep mouse cd4 t cell isolation kit, by STEMCELL (1 mentions) Anti cd3 cd28 beads, by Miltenyi Biotec (1 mentions) Sb203580, by Apexbio (1 mentions) Nsc74859, by Apexbio (1 mentions) Gsk690693, by Apexbio (1 mentions) Tgf β, by Thermo Fisher Scientific (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Edta vacutainer tube, by BD (1 mentions) Human cd8 t cell isolation kit, by Miltenyi Biotec (1 mentions) Human t cell enrichment cocktail, by STEMCELL (1 mentions) Interleukin 2 (il 2), by Thermo Fisher Scientific (1 mentions) Emetine, by Merck Group (1 mentions) Amphotericin b, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Ficoll (25 citations) Ficoll gradient (4 citations) Ficoll gradient centrifugation (3 citations) Ficoll density gradient (2 citations)

5 protocols using ficoll density gradient centrifugation

Modulating T Cell Responses in SLE

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood mononuclear cells (PBMCs) were isolated from the blood samples of SLE patients and healthy controls by Ficoll density gradient centrifugation (STEMCELL Technologies, Vancouver, CA). Human CD4⁺ or naïve CD4⁺ T cells were purified from fresh PBMCs by using the EasySep Human CD4⁺ or Naïve CD4⁺ T Cell Isolation kits (STEMCELL Technologies), stimulated with anti-CD3/CD28 beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Then, CD3/CD28 beads-stimulated naïve CD4⁺ T cells were treated with tofacitinib, STAT3 inhibitor (NSC74859), AKT inhibitor (GSK690693), or p38 MAPK inhibitor (SB203580) for 24 h. NSC74859, GSK690693, and SB203580 were purchased from ApexBio (Houston, TX, USA). Tofacitinib was added at the determined concentrations (0.1 µM, 1 µM, and 10 µM) for 24 h. In some experiments, CD4⁺ T cells were stimulated with anti-CD3/CD28 beads in the presence of recombinant cytokines, such as TGF-β (5 ng/ml; PeproTech, Rocky Hill, NJ) and IL-6 (100 ng/ml; PeproTech).
Spleens were isolated, and the erythrocytes in splenocyte suspensions were lysed with red cell lysate. Mouse CD4⁺ T cells were purified from splenocytes by using the EasySep Mouse CD4⁺ T Cell Isolation kit (STEMCELL Technologies) and processed for real-time PCR or Western blotting analysis.

Yan Q., Chen W., Song H., Long X., Zhang Z., Tang X., Chen H., Lin H, & Sun L. (2021). Tofacitinib Ameliorates Lupus Through Suppression of T Cell Activation Mediated by TGF-Beta Type I Receptor. Frontiers in Immunology, 12, 675542.

+ Open protocol

+ Expand

Multiomics Profiling of Blood and Stool

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole blood (8mL) was collected by a phlebotomist by venipuncture in Vacutainer® EDTA tubes (ethylenediaminetetraacetic acid, BD, New Jersey, United States). Blood samples were immediately processed to separate plasma by centrifugation (3,000 rpm for 10 min) and cryopreserved at −80°C. Next, peripheral blood mononuclear cells (PBMC) were isolated by Ficoll density gradient centrifugation (STEMCELL technologies, Vancouver, Canada) and stored at -196°C. Stool samples were collected by each participant and processed immediately. First, stools were classified according to the Bristol Stool Chart (34 (link)), then 220 mgs of feces were aliquoted in 2mL Eppendorf LoBind tubes, and stored at -80°C.

Rosel-Pech C., Pinto-Cardoso S., Chávez-Torres M., Montufar N., Osuna-Padilla I., Ávila-Ríos S., Reyes-Terán G., Aguirre-Alvarado C., Matías Juan N.A., Pérez-Lorenzana H., Vázquez-Rosales J.G, & Bekker-Méndez V.C. (2023). Distinct fecal microbial signatures are linked to sex and chronic immune activation in pediatric HIV infection. Frontiers in Immunology, 14, 1244473.

+ Open protocol

+ Expand

Enrichment and Activation of Human T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

T cells were isolated directly from human whole blood with a human T‐cell enrichment cocktail (STEMCELL, Cat#15021) and Ficoll density gradient centrifugation (STEMCELL, Cat#07801). Next, the T cells were activated with anti–CD3/CD28 beads (Gibco, Cat#11131D) and expanded for approximately 5 days. The T cells were grown in advanced RPMI 1640 medium supplemented with 2 mmol/L l‐glutamine, 10% FBS, and recombinant human interleukin‐2 (IL‐2; 30 U/mL, Gibco, Cat#PHC0026). CD8⁺ T cells were isolated directly from human whole blood with a human CD8⁺ T‐cell Isolation Kit (Miltenyi, Cat#130‐096‐495) and Ficoll density gradient centrifugation (STEMCELL, Cat#07801).

Wang C., Weng M., Xia S., Zhang M., Chen C., Tang J., Huang D., Yu H., Sun W., Zhang H, & Lai M. (2020). Distinct roles of programmed death ligand 1 alternative splicing isoforms in colorectal cancer. Cancer Science, 112(1), 178-193.

+ Open protocol

+ Expand

Monocyte-derived Dendritic Cell Generation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood mononuclear cells (PBMCs) were isolated from 50 mL of blood by gradient separation on Ficoll density gradient centrifugation (STEMCELL Technologies, Grenoble, France). Monocytes used to generate MD-DCs were purified by magnetic cell sorting using anti-CD14 monoclonal antibody (mAb)-coated beads (BD IMag, Le Pont de Claix, France). Sorted monocytes were morphologically homogeneous with 99% of CD14⁺ cells, as determined by flow cytometry.
Monocytes were further cultured for 6 days in 24-well plates (400,000 cells/500 μL) in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% heat-inactivated fetal calf serum, 100 U/mL penicillin, 100 μg/mL streptomycin, 500 U/mL recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF) and 500 U/mL rhIL-4 (AbCys, Paris, France). Then, the MD-DCs were stimulated or not with lipopolysaccharide (LPS) from Escherichia coli (LPS, Sigma-Aldrich, St Louis, MO, USA) at a concentration of 100 ng/mL for the last 6 or 24 hours of culture (further referred to as time points H0, H6 and H24).
CD4⁺ T cells were purified from PBMCs from two unrelated healthy donors by magnetic cell sorting using anti-CD4 monoclonal antibody (mAb)-coated beads (BD IMag), and stored frozen until used for mixed lymphocyte reaction (MLR).

Talpin A., Costantino F., Bonilla N., Leboime A., Letourneur F., Jacques S., Dumont F., Amraoui S., Dutertre C.A., Garchon H.J., Breban M, & Chiocchia G. (2014). Monocyte-derived dendritic cells from HLA-B27+ axial spondyloarthritis (SpA) patients display altered functional capacity and deregulated gene expression. Arthritis Research & Therapy, 16(4), 417.

+ Open protocol

+ Expand

Establishing B-LCLs and Inhibiting NMD

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBMC were isolated from 30 ml of freshly drawn blood by Ficoll density-gradient centrifugation (StemCell Technologies). B-lymphoblastoid cell lines (B-LCLs) were established from Epstein-Barr virus (EBV) transformed B lymphocytes according to the standard protocol⁵⁹, and grown in an atmosphere with 5% CO₂ at 37 °C in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum, penicillin (100 units/ml), streptomycin (100 μg/ml) and amphotericin B (Invitrogen). B-LCLs were cultured at 5 × 10⁵/ml. For the inhibition of Nonsense Mediated Decay (NMD), cells were transferred to a new flask and kept in culture for 7 h in the presence or absence of emetine (Sigma) at concentration of 100 μg/ml.

Paladini F., Fiorillo M.T., Vitulano C., Tedeschi V., Piga M., Cauli A., Mathieu A, & Sorrentino R. (2018). An allelic variant in the intergenic region between ERAP1 and ERAP2 correlates with an inverse expression of the two genes. Scientific Reports, 8, 10398.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!