S7946

RAEC were divided into five groups: a control group, a 2.5% TSW group, a 5% TSW group, a 10% TSW group, and a CAY10585 (an HIF-1α inhibitor) group. The control group cells were treated with 10% blank serum (0% TSW) for 24 h. The cells of the 2.5%, 5%, and 10% TSW groups were treated with 2.5% TSW-containing serum, 5% TSW-containing serum, and 10% TSW-containing serum, respectively, for 24 h. We calculated the mixture of different volumes of TSW-containing serum and blank serum to ensure that the concentration of blank serum used in all groups was 10%. The cells of the CAY10585 group were treated with 10% TSW-containing serum in combination with 20 μM CAY10585 (cat# ab144422, Abcam, USA) for 24 h. To further verify the effects of TSW-containing serum, the cells were divided into six groups consisting of the control group, the 2.5% TSW group, the 5% TSW group, the 10% TSW group, the CAY10585 group, and a KC7F2 group (another HIF inhibitor-1, cat# S7946, Selleck, USA). The cells of the KC7F2 group were treated the same as that of the CAY10585 group.