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Microtainer tubes with dipotassium edta

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Microtainer tubes with dipotassium EDTA are a type of laboratory equipment used for the collection and storage of blood samples. These tubes contain the anticoagulant dipotassium EDTA, which helps prevent the blood from clotting during the collection and handling process.

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Lab products found in correlation

Rbc lysis buffer, by Thermo Fisher Scientific (2 mentions) Phorbol myristate acetate (pma), by Merck Group (2 mentions) Ionomycin, by Merck Group (2 mentions)

Spelling variants of this product

EDTA tubes (290 citations) BD Microtainer (240 citations) Microtainer tubes (239 citations) EDTA Microtainer tubes (12 citations) Microtainer EDTA tubes (8 citations) EDTA microtainers (7 citations) Dipotassium EDTA (4 citations) Dipotassium EDTA tubes (2 citations) BD Microtainer tube with Dipotassium EDTA (2 citations)

2 protocols using microtainer tubes with dipotassium edta

1

Infant Blood Cytokine Profiling

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Fresh blood from infants was collected in Microtainer tubes with dipotassium EDTA (BD Diagnostics, Sparks Glencoe, MD) during the first, second and fourth week of age, at the same time a clinical sample was needed. Within 4 hours of collection, blood was diluted with an equal volume of complete medium (RPMI 1640, supplemented with 10% heat-inactivated fetal calf-serum, HEPES, Glutamine) and divided into two equal aliquots: one left untreated and the other stimulated with PMA (50ng/ml) (Sigma-Aldrich, St. Louis, MO) and ionomycin (1ug/ml) (EMD Millipore, Billerica, MA). The cultures were incubated for 4h at 37°C. Red blood cells were lysed using 1X RBC Lysis Buffer according to the manufacturer’s instructions (eBioscience, San Diego, CA) and the leukocytes were pelleted and stored at −80°C until the extraction of mRNA.
Jackson C.M., Wells C.B., Tabangin M.E., Meinzen-Derr J., Jobe A.H, & Chougnet C.A. (2016). Pro-inflammatory immune responses in leukocytes of premature infants exposed to maternal chorioamnionitis or funisitis. Pediatric research, 81(2), 384-390.
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2

Infant Blood Cytokine Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fresh blood from infants was collected in Microtainer tubes with dipotassium EDTA (BD Diagnostics, Sparks Glencoe, MD) during the first, second and fourth week of age, at the same time a clinical sample was needed. Within 4 hours of collection, blood was diluted with an equal volume of complete medium (RPMI 1640, supplemented with 10% heat-inactivated fetal calf-serum, HEPES, Glutamine) and divided into two equal aliquots: one left untreated and the other stimulated with PMA (50ng/ml) (Sigma-Aldrich, St. Louis, MO) and ionomycin (1ug/ml) (EMD Millipore, Billerica, MA). The cultures were incubated for 4h at 37°C. Red blood cells were lysed using 1X RBC Lysis Buffer according to the manufacturer’s instructions (eBioscience, San Diego, CA) and the leukocytes were pelleted and stored at −80°C until the extraction of mRNA.
Jackson C.M., Wells C.B., Tabangin M.E., Meinzen-Derr J., Jobe A.H, & Chougnet C.A. (2016). Pro-inflammatory immune responses in leukocytes of premature infants exposed to maternal chorioamnionitis or funisitis. Pediatric research, 81(2), 384-390.
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