Human coronary artery endothelial cells (HCAECs) were cultured at 37° C in 5% CO2. Cells were grown in EBM-2 supplemented (EGM-2-MV) medium (Lonza) without antibiotics. Experiments were performed between passages five and eight and with cells 70 to 80% confluent. Treatments with adenosine-2’,3’-dialdehyde (ADA) (Sigma) were performed for 48 h at 20 μM. Tumor necrosis factor-α (TNF-α) treatments (10 ng/mL; R&D systems) were performed for 24h.
Adenosine 2 3 dialdehyde ada
Manufactured by Merck Group
Sourced in United States
Adenosine-2',3'-dialdehyde (ADA) is a laboratory reagent used in biochemical research. It is a stable analog of the natural compound adenosine. ADA serves as a substrate for various enzymes involved in nucleotide metabolism.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using adenosine 2 3 dialdehyde ada
1
Investigating HCAEC Responses to ADA and TNF-α
2
HUVEC Culture and Treatments
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human umbilical vein endothelial cells (HUVECs) obtained from Lonza (Walkersville, MD, USA) were cultured at 37 °C in 5% CO2. Cells were grown in EGM-2 supplemented medium (Lonza). Experiments were performed between passages two and five and with cells 70% to 80% confluent. Treatments with adenosine-2′,3′-dialdehyde (ADA; Sigma-Aldrich, St. Louis, MO, USA) were performed for 48 h at 20 μM as previously optimized [54 (link)]. Tumor Necrosis Factor-α (TNF-α) treatments (10 ng/mL; Sigma) were performed for 24 h.
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