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2 protocols using oil red egn

1

Polymer Hydrogel Synthesis and Characterization

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A PSaMA sodium salt solution
13% (Mw 350,000), PScMA partial isobutyl
ester (Mw 65,000 g·mol–1), polyethylene glycol (PEG) (Mw 200
g·mol–1, PEG 200; Mw 400 g·mol–1, PEG 400; Mw 600 g·mol–1, PEG 600; Mw 1500 g·mol–1, PEG 1500; Mw 2000 g·mol–1, PEG 2000),
and polyethyleneimine (PEI), branched (Mn 600 g·mol–1, PEI 600) were purchased from
Sigma-Aldrich. N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), atenolol, atrazine, bezafibrate,
bisphenol A, bromothymol blue, naproxen, phenolphthalein, sulphamethoxazole,
oil red EGN, n-hexadecane, magnesium sulfate, magnesium
chloride, sodium sulfate, sodium dodecyl sulfate (SDS), sodium hydroxide,
glacial acetic acid, sodium phosphate monobasic dihydrate, phosphoric
acid 85%, and hydrochloric acid 37% were bought from Sigma-Aldrich.
Ethanol of 100% technical grade was bought from Boom B.V. n-Hexane 99+% was purchased from Acros Organics. Sodium
chloride (Sanal P) was received from AkzoNobel. Deionized water (DI,
1.0 μS·cm–1) was used for the preparation
of coagulation baths and Milli-Q water (Millipore, 0.6 μS·cm–1) was used to prepare solutions. The PSaMA solution
was dried for 16 h at 100 °C to obtain the solid polymer which
was used without further purification. All other chemicals were used
as received.
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2

Fabrication of Microfluidic Capacitive Sensors

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PS (35 kDa), PVP (10 kDa), Oil red EGN, 2-butanone (> 99%), and propylene glycol monomethyl ether acetate (PGMEA, ≥ 99.5%) were purchased from Sigma-Aldrich and were used as received. For parylene coatings, Parylene C dimer (Specialty Coating System, USA) and trichlorosilane (Aldrich Chemistry) were used as received. Sprays were conducted from pure ethanol (KOPTEC, 200 proof pure ethanol). SU-8 resin (EPON) was purchased and used as received for electrode masks. Ti/Pt electrodes were prepared on glass substrates via photolithography and acetone lift-off. They consist of two identical square pads that each branch into 225 μm and then 50 μm wide segments. Each segment is formed parallel to the other such that the 50 μm segment pair acts as a capacitor. The metal layers were deposited such that the platinum interfaces with the SU-8 mask layer and the titanium acts as an adhesion layer in contact with the glass substrate.
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