Feeder-free hiPSCs (iPS-DF19-9-7T and iPS-DF6-9-9T, WiCell) were cultured on hESC-qualified matrigel (Corning) with StemMACS iPSC-Brew XF media (Miltenyi Biotec). Conditions to differentiate hiPSCs into CMs in this work were tested against an established WNT-based cardiogenesis protocol (Lian et al., 2012 (link), 2013 (link)). Briefly, confluent hiPSCs were differentiated in RPMI 1640 media supplemented with B-27 supplement minus insulin (ThermoFisher Scientific) from days 0–7. To promote cardiogenesis, RPMI media was supplemented with 6 μM GSK3 inhibitor CHIR99021 (Tocris), and 5 μM WNT inhibitor IWR1 (Tocris) from differentiation days 0–2 and 3–5, respectively. Small molecule Nodal inhibitor SB431542 (Tocris) was added either at days 0–2, 3–5 or 5–7 as specified in the results section.
Stemmacs ipsc brew xf media
Manufactured by Miltenyi Biotec
StemMACS iPSC-Brew XF media is a xeno-free, chemically defined medium developed for the expansion and maintenance of human induced pluripotent stem cells (iPSCs) in feeder-free culture systems.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 media, by Thermo Fisher Scientific (4 mentions)
Gsk3 inhibitor chir99021, by Bio-Techne (2 mentions)
B 27 supplement minus insulin, by Thermo Fisher Scientific (2 mentions)
Ips df6 9 9t, by WiCell (2 mentions)
Ips df19 9 7t, by WiCell (2 mentions)
Wnt inhibitor iwr1, by Bio-Techne (2 mentions)
Sb431542, by Bio-Techne (2 mentions)
Hesc qualified matrigel, by Corning (2 mentions)
2 protocols using stemmacs ipsc brew xf media
1
Feeder-free hiPSC Cardiomyocyte Differentiation
2
Feeder-free hiPSC Cardiomyocyte Differentiation
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Feeder-free hiPSCs (iPS-DF19-9-7T and iPS-DF6-9-9T, WiCell) were cultured on hESC-qualified matrigel (Corning) with StemMACS iPSC-Brew XF media (Miltenyi Biotec). Conditions to differentiate hiPSCs into CMs in this work were tested against an established WNT-based cardiogenesis protocol (Lian et al., 2012 (link), 2013 (link)). Briefly, confluent hiPSCs were differentiated in RPMI 1640 media supplemented with B-27 supplement minus insulin (ThermoFisher Scientific) from days 0–7. To promote cardiogenesis, RPMI media was supplemented with 6 μM GSK3 inhibitor CHIR99021 (Tocris), and 5 μM WNT inhibitor IWR1 (Tocris) from differentiation days 0–2 and 3–5, respectively. Small molecule Nodal inhibitor SB431542 (Tocris) was added either at days 0–2, 3–5 or 5–7 as specified in the results section.
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