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Dcfh da fluorescent dyes

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DCFH-DA (2',7'-Dichlorodihydrofluorescein diacetate) is a non-fluorescent, cell-permeable compound that is widely used as a fluorescent indicator for the detection of reactive oxygen species (ROS) in biological systems. Upon cellular uptake, DCFH-DA is hydrolyzed by intracellular esterases to yield 2',7'-dichlorodihydrofluorescein (DCFH), which is then oxidized by ROS to the highly fluorescent 2',7'-dichlorofluorescein (DCF).

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DCFH-DA dye DCFH-DA Fluorescent dyes

2 protocols using dcfh da fluorescent dyes

1

Intracellular ROS Measurement via DCFH-DA

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Intracellular ROS level was determined using oxidation-sensitive DCFH-DA fluorescent dyes (Sigma). Cells were washed twice with PBS and labeled on the culture plates with DCFH-DA for 30 min at 37°C in serum-free medium. At the end of incubation, culture plates were trypsinized, resuspended in PBS and analyzed using a FACScan flow cytometer (λex =488 nm and λem 530nm band-pass filter). The mean fluorescence intensity of 10,000 cells was measured in each sample and corrected for autofluorescence from unlabeled cells.
Wang Y.J., Bian Y., Luo J., Lu M., Xiong Y., Guo S.Y., Yin H.Y., Lin X., Li Q., Chang C.C., Chang T.Y., Li B.L, & Song B.L. (2017). Cholesterol and fatty acids regulate cysteine ubiquitination of ACAT2 through competitive oxidation. Nature cell biology, 19(7), 808-819.
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2

Intracellular ROS Measurement via DCFH-DA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Intracellular ROS level was determined using oxidation-sensitive DCFH-DA fluorescent dyes (Sigma). Cells were washed twice with PBS and labeled on the culture plates with DCFH-DA for 30 min at 37°C in serum-free medium. At the end of incubation, culture plates were trypsinized, resuspended in PBS and analyzed using a FACScan flow cytometer (λex =488 nm and λem 530nm band-pass filter). The mean fluorescence intensity of 10,000 cells was measured in each sample and corrected for autofluorescence from unlabeled cells.
Wang Y.J., Bian Y., Luo J., Lu M., Xiong Y., Guo S.Y., Yin H.Y., Lin X., Li Q., Chang C.C., Chang T.Y., Li B.L, & Song B.L. (2017). Cholesterol and fatty acids regulate cysteine ubiquitination of ACAT2 through competitive oxidation. Nature cell biology, 19(7), 808-819.
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