Anti glua2

Antibodies used include anti-STEP₆₁ (catalogue SC-23892, Santa Cruz), anti-GluN2B (#14544, Cell Signaling), anti-ERK1/2 (SC-154, Santa Cruz), anti-GluA2 (#5306, Cell Signaling), anti-APP (#SC-28365, Santa Cruz), and anti-β-actin (#4967, Cell Signaling). Phosphorylation site specific antibodies used include anti-GluN2B-pTyr¹⁴⁷² which recognizes phosphorylated Tyr-1472 of GluN2B (P1516-1472, PhosphoSolutions), anti-ERK1/2-pThr²⁰²/Tyr²⁰⁴ which recognizes phosphorylated Thr²⁰²/Tyr²⁰⁴ of ERK1 and Thr¹⁸⁵/Tyr¹⁸⁷ of ERK2 (#9106, Cell Signaling), anti-GluA2-p3Y which recognizes phosphorylated Tyr⁸⁶⁹, Tyr⁸⁷³, and Tyr⁸⁷⁶ (3Y) of GluA2 (#3921S, Cell Signaling), and anti-GluA2-pY⁸⁷⁶ which recognizes phosphorylated Tyr⁸⁷⁶ of GluA2 (#4027S, Cell Signaling).

Fer-1, 3-MA were purchased from Med Chem Express Ltd. (Newark, NJ, USA). VC and DMSO were from Sinopharm chemical reagent Co, Ltd. (Shanghai, China). Anti- GluNR2B, anti-GluNR2A, anti-GluA1, anti-GluA2, anti-SYN1, anti-SYT1 and anti-PSD95 were obtained from Cell Signaling Technology (Boston, MA, USA). Anti-FPN-1, Anti-GPX4, and Anti-TFRC, anti-ACSL4, anti-4-HNE were from Abcam (Boston, MA, USA). Anti-SLC7A11, anti-β-actin, goat anti-rabbit-HRP second antibody, and MTT were purchased from Beyotime Technology (Shanghai, China). Anti-FTH1 was from Absin Technology (Shanghai, China). MDA Assay Kits and L-glutathione GSH Assay Kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Goat anti-mouse IgG was from Jackson ImmunoResearch (West Grove, PA, USA). Penicillin, fetal bovine serum (FBS), streptomycin, Dulbecco’s Modified Easle’s medium/F12 (DMEM/F12), and trypsin were purchased from Thermo Scientific (Waltham, MA, USA).