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Picoprobe free glycerol fluorometric assay kit

Manufactured by Abcam

The PicoProbe Free Glycerol Fluorometric Assay Kit is a biochemical assay that measures the concentration of free glycerol in a sample. The kit uses a proprietary fluorogenic probe that reacts with free glycerol, generating a fluorescent signal that can be quantified using a fluorometer or fluorescence microplate reader.

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2 protocols using picoprobe free glycerol fluorometric assay kit

1

Glycerol Assay in Whole Worms

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Glycerol assays were conducted on populations of whole worms lysed by sonication using the PicoProbe Free Glycerol Fluorometric Assay Kit (Biovision). Values were normalized to total protein using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific).
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2

Glycerol Assay and Motility Analysis in Worms

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Glycerol assays were conducted on populations of worms grown on agar as described for RT-qPCR. Samples were lysed by sonication and processed as described previously using the PicoProbe Free Glycerol Fluorometric Assay Kit (Biovision K643-100) [31 (link),39 ]. Values were normalized to total protein using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific 23227). For acute motility assays, worms were transferred to high NaCl agar by chunking and motility was scored as described previously [39 ].
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