NIH 3T3 as well as Src−/−, Yes−/−, and Fyn−/− (SYF) mouse embryonic fibroblasts (MEFs) were grown in DMEM supplemented with 10% FBS, 1% L-Glutamine, and Pen/Strep. Cells were maintained on Thermo Scientific Nunc Cell Culture Treated Flasks with Filter Caps and grown at 37°C with 5% CO2. These same conditions were used for Lenti-X-293T cells shown in Figure S2 . JCAM 2.5 Jurkat cells were grown in RPMI medium supplemented with 10% FBS, 1% L-Glutamine, and Pen/Strep and grown in the same conditions as noted above. Cell lines were obtained from repositories (ATCC) or from the labs that generated the cell lines (for JCAM2.5 Jurkat cells) and were not independently authenticated.
Nunc cell culture treated flasks with filter caps
Manufactured by Thermo Fisher Scientific
Sourced in United States
Nunc Cell Culture Treated Flasks with Filter Caps are sterile, single-use flasks designed for cell culture applications. They feature a treated polystyrene surface to enhance cell attachment and growth. The flasks are equipped with a filter cap that allows gas exchange while maintaining a sterile environment.
Automatically generated - may contain errors
Lab products found in correlation
Glutamine, by Merck Group (1 mentions)
Fetal calf serum (fcs), by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
4 protocols using nunc cell culture treated flasks with filter caps
1
Cell Culture Protocol for Various Cell Lines
2
Culturing Murine Keratinocytes with H2B-RFP
3
Transient Transfection for IgG Expression
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Transformed Human Embryo Kidney monolayer epithelial cells (PEAK cells; Edge Bio, La Jolla, CA, USA) were maintained in 5% CO2 at 37 °C in a humidified atmosphere in DMEM (Invitrogen, Carlsbad, CA, USA) containing 10% FCS (Sigma-Aldrich, St Louis, MO, USA) and supplemented with 2 mM glutamine (Sigma-Aldrich, St Louis, MO, USA), referring to complete DMEM. One day before transfection, confluent cells were splited 1:4 in T175 flasks (Nunc™ Cell Culture Treated Flasks with Filter Caps, Thermo Scientific, Waltham, MA, USA) in order to have cells in the exponential growth phase. Transient transfections were performed using a mix containing 30 µg of DNA and 42 µL of Lipofectamine 2000 transfection reagent (Invitrogen, Carlsbad, CA, USA) in 1.4 mL of DMEM for 107 cells per T175 flask in 50 mL of complete DMEM.
IgG expression was measured using the Octet RED96 instrument with protein A-coated biosensors (Pall ForteBio, Menlo Park, CA, USA). According to antibody concentration, supernatants were harvested 7 to 10 days after transfection.
IgG expression was measured using the Octet RED96 instrument with protein A-coated biosensors (Pall ForteBio, Menlo Park, CA, USA). According to antibody concentration, supernatants were harvested 7 to 10 days after transfection.
4
Cell Culture Protocol for Various Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NIH 3T3 as well as Src−/−, Yes−/−, and Fyn−/− (SYF) mouse embryonic fibroblasts (MEFs) were grown in DMEM supplemented with 10% FBS, 1% L-Glutamine, and Pen/Strep. Cells were maintained on Thermo Scientific Nunc Cell Culture Treated Flasks with Filter Caps and grown at 37°C with 5% CO2. These same conditions were used for Lenti-X-293T cells shown in Figure S2 . JCAM 2.5 Jurkat cells were grown in RPMI medium supplemented with 10% FBS, 1% L-Glutamine, and Pen/Strep and grown in the same conditions as noted above. Cell lines were obtained from repositories (ATCC) or from the labs that generated the cell lines (for JCAM2.5 Jurkat cells) and were not independently authenticated.
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