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Filamentous actin f actin specific alexa fluor 488 conjugated phalloidin

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Filamentous actin (F-actin)-specific Alexa Fluor 488-conjugated Phalloidin is a fluorescently labeled compound used to specifically stain and visualize actin filaments in cells. It binds tightly and selectively to F-actin, allowing for the detection and localization of this cytoskeletal component.

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Lab products found in correlation

Cell culture reagents, by Thermo Fisher Scientific (2 mentions) Cy3 conjugated anti mouse alpha smooth muscle actin antibody, by Merck Group (2 mentions) Anti glucagon cat, by Abcam (2 mentions) Anti insulin cat, by Abcam (2 mentions) Goat anti guinea pig igg h l conjugated alexa fluor 488 ca ab150185, by Abcam (2 mentions) Goat anti mouse igg h l conjugated alexa fluor 594 ca ab150116, by Abcam (2 mentions) Anti human c peptide cat gn 1d4, by Developmental Studies Hybridoma Bank (2 mentions) Nkx 6.1 cat f55a12, by Developmental Studies Hybridoma Bank (2 mentions)

Close spelling variants of this product

Alexa Fluor 488 (6806 citations) Alexa 488 (1863 citations) Alexa Fluor 488-conjugated phalloidin (250 citations) Alexa 488 phalloidin (170 citations) F-actin (133 citations) Alexa 488-conjugated phalloidin (83 citations) Phalloidin-488 (81 citations) Alexa Fluor–conjugated phalloidin (44 citations) Alexa Fluor phalloidin (43 citations) Alexa Fluors (21 citations)

2 protocols using filamentous actin f actin specific alexa fluor 488 conjugated phalloidin

1

Comprehensive Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals were obtained from Sigma-Aldrich (St. Louis, MO) and cell culture reagents from Life Technologies (Grand Island, NY), unless otherwise noted. Antibodies: Alexa Fluor 488-conjugated anti-mouse CD68 (Cat. #137012, Clone FA-11) and Alexa Fluor 647-conjugated anti-mouse Ly-6G/Ly-6C (Gr-1) (Cat. #137012, Clone RB6-8C5) were purchased from BioLegend Inc. (San Diego, CA). Cy3-conjugated anti-mouse alpha smooth muscle actin antibody was purchased from Sigma Aldrich (St. Louis MO). Filamentous actin (F-actin)-specific Alexa Fluor 488-conjugated Phalloidin was purchased from Life Technologies (Grand Island, NY). Anti-Glucagon cat# ab82270, Anti-insulin cat# ab7842, Goat Anti-Guinea pig IgG H&L conjugated Alexa Fluor® 488 ca#ab150185, and Goat Anti-Mouse IgG H&L conjugated Alexa Fluor® 594 ca#ab150116 were purchased from abcam (Cambridge, MA). Anti human C-peptide cat #GN-1D4, and NKX 6.1 cat # F55A12 was purchased from Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, Iowa). A sampling of spheres used in study were submitted for endotoxin testing by a commercial vendor (Charles River, Wilmington, MA) and the results showed that spheres contained < 0.05 EU/ml of endotoxin levels.
Vegas A.J., Veiseh O., Gürtler M., Millman J.R., Pagliuca F.W., Bader A.R., Doloff J.C., Li J., Chen M., Olejnik K., Tam H.H., Jhunjhunwala S., Langan E., Aresta-Dasilva S., Gandham S., McGarrigle J., Bochenek M.A., Hollister-Lock J., Oberholzer J., Greiner D.L., Weir G.C., Melton D.A., Langer R, & Anderson D.G. (2016). Long term Glycemic Control Using Polymer Encapsulated, Human Stem-Cell Derived β-cells in Immune Competent mice. Nature medicine, 22(3), 306-311.
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2

Comprehensive Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals were obtained from Sigma-Aldrich (St. Louis, MO) and cell culture reagents from Life Technologies (Grand Island, NY), unless otherwise noted. Antibodies: Alexa Fluor 488-conjugated anti-mouse CD68 (Cat. #137012, Clone FA-11) and Alexa Fluor 647-conjugated anti-mouse Ly-6G/Ly-6C (Gr-1) (Cat. #137012, Clone RB6-8C5) were purchased from BioLegend Inc. (San Diego, CA). Cy3-conjugated anti-mouse alpha smooth muscle actin antibody was purchased from Sigma Aldrich (St. Louis MO). Filamentous actin (F-actin)-specific Alexa Fluor 488-conjugated Phalloidin was purchased from Life Technologies (Grand Island, NY). Anti-Glucagon cat# ab82270, Anti-insulin cat# ab7842, Goat Anti-Guinea pig IgG H&L conjugated Alexa Fluor® 488 ca#ab150185, and Goat Anti-Mouse IgG H&L conjugated Alexa Fluor® 594 ca#ab150116 were purchased from abcam (Cambridge, MA). Anti human C-peptide cat #GN-1D4, and NKX 6.1 cat # F55A12 was purchased from Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, Iowa). A sampling of spheres used in study were submitted for endotoxin testing by a commercial vendor (Charles River, Wilmington, MA) and the results showed that spheres contained < 0.05 EU/ml of endotoxin levels.
Vegas A.J., Veiseh O., Gürtler M., Millman J.R., Pagliuca F.W., Bader A.R., Doloff J.C., Li J., Chen M., Olejnik K., Tam H.H., Jhunjhunwala S., Langan E., Aresta-Dasilva S., Gandham S., McGarrigle J., Bochenek M.A., Hollister-Lock J., Oberholzer J., Greiner D.L., Weir G.C., Melton D.A., Langer R, & Anderson D.G. (2016). Long term Glycemic Control Using Polymer Encapsulated, Human Stem-Cell Derived β-cells in Immune Competent mice. Nature medicine, 22(3), 306-311.
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