Western blot analysis was performed as described previously [33 (link)] using the following antibodies: Mouse anti-human Caspase-8 (Enzo, Farmingdale, NY, USA), rabbit anti-GFP (Clontech), and mouse anti-β-Actin (Sigma-Aldrich). Goat anti-mouse IgG or goat anti-rabbit IgG conjugated to horseradish peroxidase (Santa Cruz Biotechnologies, Dallas, TX, USA) and enhanced chemiluminescence (Amersham Bioscience, Freiburg, Germany) or infrared dye-labeled secondary antibodies and infrared imaging (Odyssey Imaging System, LI-COR Bioscience, Bad Homburg, Germany) were used for detection.
Goat anti mouse igg or goat anti rabbit igg conjugated to horseradish peroxidase
Manufactured by Santa Cruz Biotechnology
Sourced in Germany
Goat anti-mouse IgG or goat anti-rabbit IgG conjugated to horseradish peroxidase is a secondary antibody used in Western blotting, ELISA, and other immunoassays. The antibody binds to the primary antibody, which is specific to the target protein, and the horseradish peroxidase enzyme can then be used to catalyze a colorimetric or chemiluminescent reaction for detection and quantification of the target protein.
Automatically generated - may contain errors
Lab products found in correlation
Odyssey imaging system, by LI COR (3 mentions)
Mouse anti β actin, by Merck Group (3 mentions)
Enhanced chemiluminescence, by Cytiva (2 mentions)
Mouse anti gapdh, by HyTest (2 mentions)
Mouse anti human caspase 8, by Enzo Life Sciences (1 mentions)
Rabbit anti gfp, by Takara Bio (1 mentions)
Rabbit anti phospho stat1, by Cell Signaling Technology (1 mentions)
Mouse anti irf1, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti phospho mlkl, by Cell Signaling Technology (1 mentions)
Mouse anti vinculin, by Merck Group (1 mentions)
Mouse anti gapdh antibody, by Cell Signaling Technology (1 mentions)
Ecl western blotting detection reagent, by Cytiva (1 mentions)
Rabbit anti phospho s6 ribosomal protein ser235 236 antibody, by Cell Signaling Technology (1 mentions)
Rabbit anti phospho akt ser473 antibody, by Cell Signaling Technology (1 mentions)
Hybond ecl nitrocellulose membrane, by Cytiva (1 mentions)
Mouse anti gpx4, by R&D Systems (1 mentions)
4 protocols using goat anti mouse igg or goat anti rabbit igg conjugated to horseradish peroxidase
1
Western Blot Analysis of Caspase-8 and GFP
2
Western Blot Analysis of Protein Expression
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Western blot analysis was performed as described previously [25] (link) using the following antibodies: Mouse anti-STAT1 (Cell Signaling, Beverly, MA, USA), rabbit anti-phospho-STAT1 (Cell Signaling), mouse anti-IRF1 (Santa Cruz Biotechnologies, Santa Cruz, CA, USA), rabbit anti-MLKL (GeneTex, Inc., Irvine, CA, USA), rabbit anti-phospho-MLKL (Cell Signaling) mouse anti-GAPDH (HyTest, Turku, Finland), mouse anti-β-Actin (Sigma-Aldrich), mouse anti-Vinculin (Sigma-Aldrich). Goat anti-mouse IgG or goat anti-rabbit IgG conjugated to horseradish peroxidase (Santa Cruz Biotechnologies) and enhanced chemiluminescence (Amersham Bioscience, Freiburg, Germany) or infrared dye-labeled secondary antibodies and infrared imaging (Odyssey Imaging System, LI-COR Bioscience, Bad Homburg, Germany) were used for detection. Representative blots of at least two independent experiments are shown. Protein expressions of Western blots were quantified using ImageJ 1.52e and normalized to β-Actin protein expression.
3
Western Blot Analysis of Akt and S6 Phosphorylation
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Western blot analysis was performed as previously described [25 (link)]. Briefly, after lysis and determination of protein concentration, samples were separated on a 12% SDS-polyacrylamide electrophoresis gel and transferred onto a Hybond ECL nitrocellulose membrane (Amersham Biosciences, Freiburg, Germany). Proteins were visualized by ECL western blotting detection reagents (Amersham Biosciences), according to manufacturer's instruction, and following antibodies were used: rabbit anti–phospho-Akt (Ser473) antibody (Cell Signaling), rabbit anti-phosph-Akt (Thr308) antibody (Cell Signaling), mouse anti-Akt antibody (Bioscience, Heidelberg, Germany), rabbit anti–phospho-S6 ribosomal protein (Ser235/236) antibody (Cell Signaling), rabbit anti–S6 ribosomal protein antibody (Cell Signaling), or mouse anti-GAPDH antibody (Cell Signaling) followed by goat-anti-mouse IgG or goat-anti-rabbit IgG conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Heidelberg, Germany).
4
Western Blot Analysis of GPX4 and RIP3
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Western blot analysis was performed as described previously [25 (link)] using the following antibodies: mouse anti-GPX4 (R&D Systems, Inc., Wiesbaden, Germany), rabbit anti-RIP3 (Imgenex, San Diego, CA, USA), mouse anti-β-Actin (Sigma-Aldrich) or mouse anti-GAPDH (HyTest, Turku, Finland). Goat anti-mouse IgG or goat anti-rabbit IgG conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Santa Cruz, CA) and enhanced chemiluminescence (Amersham Bioscience, Freiburg, Germany) or infrared dye-labeled secondary antibodies and infrared imaging (Odyssey Imaging System, LI-COR Bioscience, Bad Homburg, Germany) were used for detection. Representative blots of at least two independent experiments are shown.
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