Cells were lysed in RIPA lysis buffer (Beyotime), separated by SDS‐PAGE, and transferred onto PVDF membranes (Millipore). Membranes were blocked with 5% defatted milk for 1 h and incubated with anti‐ICAT (A7122, 1:2000, ABclonal), anti‐ICAT (ab129011, 1:2000, Abcam), anti‐JUP (381,616, 1:2000, ZEN‐Bioscience), anti‐GAPDH (10494‐AP, 1:5000, Proteintech Group), anti‐phospho‐P65 (p‐P65) (AF5881, 1:1000, Beyotime), or anti‐P65 (AF0246, 1:1000, Beyotime) primary antibodies at 4°C for 10 h. The membranes were washed thrice with TBST for 5 min each and incubated with horseradish peroxidase‐conjugated secondary antibodies to rabbit (#820112, 1:2000, EarthOx) or mouse (#620112, 1:2000, EarthOx) for 1 h. The signals were detected using a chemiluminescence system (Bio‐Rad).
Ab129011
Manufactured by Abcam
Sourced in United Kingdom
Ab129011 is a laboratory reagent. It is a Rabbit polyclonal antibody that recognizes an epitope within the C-terminus of human XRCC1 protein. The antibody can be used in various applications to detect XRCC1 expression.
Automatically generated - may contain errors
Lab products found in correlation
Ripa lysis buffer, by Beyotime (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Chemiluminescence system, by Bio-Rad (1 mentions)
A7122, by ABclonal (1 mentions)
Af0246, by Beyotime (1 mentions)
Hrp conjugated anti mouse igg, by Cell Signaling Technology (1 mentions)
Hrp conjugated anti rabbit igg, by Cell Signaling Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Mg132, by Merck Group (1 mentions)
Ab101468, by Abcam (1 mentions)
Ab191071, by Abcam (1 mentions)
Ab239366, by Abcam (1 mentions)
Ab51608, by Abcam (1 mentions)
Ab115730, by Abcam (1 mentions)
Alexa fluor 647 conjugated anti rat igg, by Cell Signaling Technology (1 mentions)
2 protocols using ab129011
1
Western Blot Analysis of ICAT, JUP, and P65
2
Isolation and Characterization of Bruceine D
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bruceine D was isolated from the fruits of Brucea javanica (Linn.) Merr in our laboratory16 . For in vitro experiment, BD was dissolved in 100% DMSO (Sigma, 01934-1L) at a final stock concentration of 50 mmol/L and stored at −20 °C as single used aliquot. The antibodies for GAPDH (5174s, 1:1000), β-actin (3700s, 1:1000), HIF-1α (36169s, 1:1000), β-catenin (8480s, 1:1000), PKM2 (4053T, 1:1000), HK2 (2867T, 1:1000), LDHA (3582T, 1:1000), Cyt c (11940T, 1:1000), HRP-conjugated anti-rabbit IgG (1:5000), HRP-conjugated anti-mouse IgG (1:5000), and Alexa Fluor 647 conjugated-anti-rat IgG (4418s, 1:1000) were purchased from Cell signaling technology (CST, Danvers, MA, USA). Primary antibodies for GLUT1 (ab115730, 1:1000), GLUT3 (ab191071,1:1000), HIF-1β (ab239366, 1:1000), ICAT (ab129011, 1:1000), HIF-1α (ab51608, 1:1000), and recombinant ICAT (ab101468) were both obtained from Abcam (Cambridge, UK). MCT4 (sc-376140, 1:1000) mouse IgG (sc-2025, 1:50) was procured from Santa Cruz (OR, USA). Recombinant human ICAT protein (ab101468) was obtained from Abcam (Cambridge, UK). Cycloheximide (Sigma, 5087390001) and MG132 (Sigma, SML1135) was obtained from Sigma (Shanghai, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!