Sbc 3

We used 9 SCLC cell lines (SBC‐3, SBC‐5, MS‐1, RERF‐LC‐MA, H69, H82, H209, H592 and H1688), with SBC‐3, SBC‐5, MS‐1 and RERF‐LC‐MA obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka. Japan), and H69, H82, H209 and H1688 obtained from the American Type Culture Collection (Manassas, VA, USA). H592 was a gift from Dr Hirotoshi Dosaka‐Akita (Department of Medical Oncology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Hokkaido, Japan). SBC‐3, SBC‐5 and RERF‐LC‐MA were maintained in minimum essential medium, and MS‐1, H82, H209, H592 and H1688 were maintained in RPMI 1640 medium supplemented with 10% FBS at 37°C and 5% CO₂.

Human LUAD (HCC827), SCC (H520, H446, and SK-MES-1), and LCC (H1299) cell lines were purchased from the American Type Culture Collection. Human SCLC cell lines (LK-2 and SBC-3) were purchased from the Japanese Collection of Research Bioresources Cell Bank and validated by short tandem repeat profiling (National Institute of Biomedical Innovation). All cell lines were routinely tested for Mycoplasma and confirmed to have no contamination using a LookOut Kit (MP0035; Sigma–Aldrich) according to the manufacturer’s instructions. H1299, HCC827, H520, H446, and LK-2 were maintained in RPMI1640 (187-02705 for H520 and H446, FUJIFILM Wako; R8758 for LK-2, Sigma–Aldrich). SK-MES-1 and SBC-3 were maintained in Eagle’s minimal essential medium (M4655; Sigma–Aldrich). All media contained 10% fetal bovine serum (10270106; Sigma–Aldrich) and 100 U/ml penicillin and 10 μg/ml streptomycin (P4333; Sigma–Aldrich). Cells were cultured at 37 °C in a 5% CO₂ atmosphere.