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Epz015666

Manufactured by MedChemExpress
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EPZ015666 is a laboratory reagent produced by MedChemExpress. It is a chemical compound used for research purposes. The core function of EPZ015666 is to serve as a tool for scientific investigations, but a detailed description of its intended use cannot be provided in an unbiased and factual manner.

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Lab products found in correlation

Gsk3326595, by MedChemExpress (2 mentions) Gsk591, by MedChemExpress (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Prmi 1640, by Thermo Fisher Scientific (1 mentions) Cisplatin, by MedChemExpress (1 mentions) Gemcitabine, by MedChemExpress (1 mentions) Sb431542, by Thermo Fisher Scientific (1 mentions) Interleukin 6 (il 6), by Proteintech (1 mentions) Rhodamine phalloidin, by Yeasen (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Yeasen (1 mentions)

5 protocols using epz015666

1

Detailed Protocols for Drug Screening

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GSK591, GSK3326595, BRD0639, and EPZ015666 were obtained MedChemExpress LLC. PF-06939999 was purchased from Chemietek. CMP5 was obtained from Sigma. JNJ-64619178 was obtained from ChiralStar. For in vitro assays, the drugs were dissolved in dimethyl sulfoxide (DMSO) as stock solutions. For in vivo treatment, JNJ-64619178 was dissolved in 1% N-methyl-2-pyrrolidone, 5% DMSO, and 5% Tween-80 in ddI H2O.
Li B.X., David L.L., Davis L.E, & Xiao X. (2022). Protein arginine methyltransferase 5 is essential for oncogene product EWSR1-ATF1-mediated gene transcription in clear cell sarcoma. The Journal of Biological Chemistry, 298(10), 102434.
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2

Bladder Cancer Cell Lines Treated with FKA

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Bladder tumor cell lines, UMUC3 and T24, and urothelial cell line, SV-HUC-1, were acquired from the cell bank of the Chinese Academy of Sciences and cultured in high glucose DMEM or PRMI 1640 medium with 10% FBS (Gibco, Thermo Fisher Scientific, USA). All reagents [EPZ015666 (22 nM), GSK3326595 (6.2 nM), gemcitabine (7.5 ng/mL), cisplatin (100 mg/mL)] were purchased from MedChemExpress, and various concentrations FKA were used to treat UMUC3 and T24 cells.
Liu S., Liu Z., Piao C., Zhang Z., Kong C., Yin L, & Liu X. (2022). Flavokawain A is a natural inhibitor of PRMT5 in bladder cancer. Journal of Experimental & Clinical Cancer Research : CR, 41, 293.
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3

Cytokine Regulation in Immune Cells

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Phorbol 12-myristate 13-acetate (PMA; cat. no. P8139) and lipopolysaccharide (LPS; cat. no. L2630) were purchased from Sigma-Aldrich (Merck KGaA). The Luminex cytokine panel kit (cat. no. LXSAHM) was purchased from R&D Systems, Inc. Rabbit PRMT5 antibody (cat. no. 18436-1-AP) was purchased from ProteinTech Group, Inc. IL-6 (cat. no. BMS213-2) and IFN-γ-induced protein 10 (IP-10) ELISA kits (cat. no. KAC2361) were purchased from Thermo Fisher Scientific, Inc. SB431542 (cat. no. S1067), which is used as an inhibitor of Smad 2/3(16 (link)), was purchased from Selleck Chemicals. NF-κB inhibitor SN50 (cat. no. SML1471) and PRMT5 inhibitor EPZ015666 were obtained from MedChemExpress.
Chai X., Wu X., He L, & Ding H. (2021). Protein arginine methyltransferase 5 mediates THP-1-derived macrophage activation dependent on NF-κB in endometriosis. Experimental and Therapeutic Medicine, 22(3), 1003.
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4

Evaluating EPZ015666 Effects on T-cell Viability

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Activated and naïve T-cells were seeded in 12-well plates at 5 × 105 cells/mL and all other lymphoid cells were seeded in 12-well plates at 1 × 105 cells/mL. EPZ015666 (MedChemExpress) or vehicle (DMSO) were added to duplicate wells at the indicated concentrations. Cells were incubated at 37°C. Cell viability was measured on days 4, 8, and 12 using Trypan blue dye exclusion. Fresh EPZ015666 or vehicle were added to the appropriate wells at the indicated concentrations every 4 days.
Ernzen K., Melvin C., Yu L., Phelps C., Niewiesk S., Green P.L, & Panfil A.R. (2023). The PRMT5 inhibitor EPZ015666 is effective against HTLV-1-transformed T-cell lines in vitro and in vivo. Frontiers in Microbiology, 14, 1101544.
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5

Antibody Validation for Cardiac Research

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The following primary antibodies were utilized in this study: Anti-HoxA9 (sc-81291), anti-αTubulin (sc-398103), anti-PRMT5 (sc-376937) and Anti-β-MHC (sc-53089) mouse monoclonal antibodies were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, United States). Anti-BNP rabbit polyclonal antibody (PAA541Ra01) was purchased by Cloud-Clone Corp (Wuhan, Hubei, China). Anti-mouse Anti-mouse IgG, HRP-linked secondary antibody (#7076P2), and Anti-mouse Anti-rabbit IgG, HRP-linked secondary antibody (#7074P2) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, United States).
ISO was acquired from TargetMol (Massachusetts, USA). Rhodamine phalloidin and 4′6-diamidino-2-phenylindole (DAPI) were purchased from YeasenBiotechCo. Ltd. (Shanghai, China). EPZ015666 was bought from MedChem Express (New Jersey, United States).
Cai S., Liu R., Wang P., Li J., Xie T., Wang M., Cao Y., Li Z, & Liu P. (2020). PRMT5 Prevents Cardiomyocyte Hypertrophy via Symmetric Dimethylating HoxA9 and Repressing HoxA9 Expression. Frontiers in Pharmacology, 11, 600627.
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