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Tristar2 lb942 luminometer

Manufactured by Berthold Technologies
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Sourced in Germany

The TriStar2 LB942 luminometer is a compact, versatile instrument designed for the detection and quantification of luminescent signals. It utilizes a photomultiplier tube (PMT) to measure the intensity of light emitted from various samples, providing accurate and sensitive results.

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Lab products found in correlation

One glo luciferase assay system, by Promega (3 mentions) Nano glo live cell assay system, by Promega (3 mentions)

Spelling variants of this product

TriStar2 (27 citations) LB942 (10 citations) TriStar2 luminometer (3 citations)

4 protocols using tristar2 lb942 luminometer

1

Gaussia Luciferase Reporter Assay

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Luciferase assay were performed 48 hr after incubation. The supernatants were collected to assess Gaussia luciferase activity using Genecopoeia Luc-Pair Renilla luciferase HS Assay Kit (GeneCopoeia) following the manufacturer’s instruction and measured on a Tristar2 LB942 luminometer (Berthold Technologies). See Quantification and statistical analysis for details on statistical comparisons.
Sanders D.W., Jumper C.C., Ackerman P.J., Bracha D., Donlic A., Kim H., Kenney D., Castello-Serrano I., Suzuki S., Tamura T., Tavares A.H., Saeed M., Holehouse A.S., Ploss A., Levental I., Douam F., Padera R.F., Levy B.D, & Brangwynne C.P. (2021). SARS-CoV-2 requires cholesterol for viral entry and pathological syncytia formation. eLife, 10, e65962.
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2

Protein-Protein Interaction Monitoring

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DNA fragments of PBI1, WRKY451–326, WRKY451–174, WRKY45175–326, WRKY45DD, PUB44 were transferred using the Gateway system with LR clonase reactions into p35S-LgBiT-T7-GW or p35S-SmBiT-T7-GW41 (link). The plasmid containing MKK4DD was described previously13 (link). The Firefly Luciferase gene under the control of CaMV 35S promoter was used as an internal control. The indicated combinations of plasmids were used to transfect rice protoplasts. After 18 h incubation at 30 °C, the activities of the Firefly and NanoLuc luciferases were measured on a TriStar2 LB942 luminometer (Berthold) using the ONE-Glo Luciferase Assay System (Promega) and the Nano-Glo Live Cell Assay System (Promega).
Ichimaru K., Yamaguchi K., Harada K., Nishio Y., Hori M., Ishikawa K., Inoue H., Shigeta S., Inoue K., Shimada K., Yoshimura S., Takeda T., Yamashita E., Fujiwara T., Nakagawa A., Kojima C, & Kawasaki T. (2022). Cooperative regulation of PBI1 and MAPKs controls WRKY45 transcription factor in rice immunity. Nature Communications, 13, 2397.
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3

Protein-Protein Interaction Assay in Rice Protoplasts

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DNA fragments of Xa11–1802, Xa11–325, Xa1312–1012, Xa11008–1802, AvrXa7 and Xoo1132 were transferred into p35S‐GW‐T7‐LgBiT, p35S‐GW‐T7‐SmBiT or p35S‐SmBiT‐GW‐T7 using the Gateway system with LR clonase reactions (Taoka et al., 2021 (link)). The Firefly Luciferase gene controlled by the CaMV 35S promoter was used as an internal control. The constructs were transfected into rice protoplasts as described above. After 18 h of incubation at 30°C, activities of the Firefly and NanoLuc luciferases were measured in a TriStar2 LB942 luminometer (Berthold, Bad Wildbad, Germany) using the ONE‐Glo Luciferase Assay System (Promega, Madison, WI, USA) and the Nano‐Glo Live Cell Assay System (Promega).
Yoshihisa A., Yoshimura S., Shimizu M., Sato S., Matsuno S., Mine A., Yamaguchi K, & Kawasaki T. (2022). The rice OsERF101 transcription factor regulates the NLR Xa1‐mediated immunity induced by perception of TAL effectors. The New Phytologist, 236(4), 1441-1454.
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4

Protein-Protein Interaction Screening in Rice

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DNA fragments of Xa1, BED, NB, LRR, AvrXa7, and Xoo1132 were transferred into p35S-LgBiT-T7-GW or p35S-SmBiT-T7-GW using the Gateway system with LR clonase reactions (Taoka et al., 2021) . The Firefly Luciferase gene controlled by the CaMV 35S promoter was used as an internal control. The constructs were transfected into rice protoplasts as described above. After 18 h incubation at 30°C, the activities of the Firefly and NanoLuc luciferases were measured in a TriStar2 LB942 luminometer (Berthold) using the ONE-Glo Luciferase Assay System (Promega) and the Nano-Glo
Live Cell Assay System (Promega).
Yoshihisa A., Yoshimura S., Shimizu M., Sato S., Mine A., Yamaguchi K., & Kawasaki T. (2021). The rice OsERF101 transcription factor regulates the NLR Xa1-mediated perception of TAL effectors and Xa1-mediated immunity.
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