For time-lapse microscopy experiments, cells were seeded into 96 well glass bottom plates with 200 μL of media two days prior to imaging (Brooks Automation Inc. 96 Well Glass Bottom Black Plates (MGB09612LGL)). A 10 μL volume of stress perturbing molecules diluted in media was added to wells at various time points before imaging. MG132 was used at final concentrations ranging from 0.06 μM to 10 μM to induce proteasome inhibition. STA9090 (Ganetespib) was used at concentrations ranging from 6.25 nM to 500 nM to induce HSP90 inhibition. Time-lapse images were taken with the GE IN Cell Analyzer 6000, Perkin-Elmer Operetta High-Content Imaging System, or Nikon Eclipse Ti Live Cell Imaging System. Cells were kept in a controlled chamber of 37°C temperature and 5% CO2. Time intervals between images varied based on experiment, ranging from 5 to 30 minutes (exact intervals are noted in the Figure legends).
In cell analyzer 6000
Manufactured by PerkinElmer
The IN Cell Analyzer 6000 is a high-content screening system designed for cell-based assays. It provides automated imaging and analysis capabilities to study cellular processes and phenotypes in a quantitative manner.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using in cell analyzer 6000
1
Time-lapse microscopy of stress response
2
Time-lapse microscopy of stress response
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For time-lapse microscopy experiments, cells were seeded into 96 well glass bottom plates with 200 μL of media two days prior to imaging (Brooks Automation Inc. 96 Well Glass Bottom Black Plates (MGB09612LGL)). A 10 μL volume of stress perturbing molecules diluted in media was added to wells at various time points before imaging. MG132 was used at final concentrations ranging from 0.06 μM to 10 μM to induce proteasome inhibition. STA9090 (Ganetespib) was used at concentrations ranging from 6.25 nM to 500 nM to induce HSP90 inhibition. Time-lapse images were taken with the GE IN Cell Analyzer 6000, Perkin-Elmer Operetta High-Content Imaging System, or Nikon Eclipse Ti Live Cell Imaging System. Cells were kept in a controlled chamber of 37°C temperature and 5% CO2. Time intervals between images varied based on experiment, ranging from 5 to 30 minutes (exact intervals are noted in the Figure legends).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!