Ambion poly a purist mag kit

Manufactured by Thermo Fisher Scientific

The Ambion® Poly(A)Purist™ MAG Kit is a magnetic bead-based system designed for the purification of polyadenylated (poly(A)) RNA from total RNA samples. The kit utilizes oligo(dT) magnetic beads to selectively capture and isolate the poly(A) RNA, which can then be eluted for downstream applications.

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Lab products found in correlation

Powerup sybr green master mix, by Thermo Fisher Scientific (2 mentions) Rneasy plus kit, by Qiagen (2 mentions) Superscript 3, by Thermo Fisher Scientific (2 mentions) Thermo scientific ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (2 mentions) Steponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions) Asteponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions)

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Poly (A) Purist MAG (4 citations)

2 protocols using ambion poly a purist mag kit

RNA Extraction and Quantification

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Total RNA was isolated employing the RNeasy Plus kit (Qiagen) according to manufacturer's protocol. Extracted RNA was reversed transcribed into cDNA using random hexamer priming and Superscript III (ThermoFisher) according to the protocol supplied by the manufacturer. For qPCR, cDNA templates were amplified, and the C_T values were quantified with PowerUp SYBR Green Master Mix (ThermoFisher), and normalized to actin as an internal control. Experiments were performed using a StepOnePlus Real-Time PCR System (Applied Biosystems). poly(A)-mRNA was isolated using Ambion^® Poly(A)Purist™ MAG Kit (ThermoFisher) according to manufacturer's protocol. Nuclear and cytoplasmic separation of RNA was performed with Thermo Scientific NE PER Nuclear and Cytoplasmic Extraction Kit (ThermoFisher) according to manufacturer's protocol. RNA in either the nuclear pellets or cytoplasmic fractions was extracted with RNeasy Plus kit (Qiagen). Total RNA extracted per fraction was quantified and used to calculate the percentage of mRNA localization for the RT-qPCR quantification. The list of primers is provided in Supplementary Data (Supplementary Table S1).

Tan Z.W., Fei G., Paulo J.A., Bellaousov S., Martin S.E., Duveau D.Y., Thomas C.J., Gygi S.P., Boutz P.L, & Walker S. (2020). O-GlcNAc regulates gene expression by controlling detained intron splicing. Nucleic Acids Research, 48(10), 5656-5669.

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Comprehensive Transcriptome Analysis Protocol

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Total RNA was isolated employing the RNeasy Plus kit (Qiagen) according to manufacturer's protocol.
Extracted RNA was reversed transcribed into cDNA using random hexamer priming and Superscript III (ThermoFisher) according to the protocol supplied by the manufacturer. For qPCR, cDNA templates were amplified, and the C T values were quantified with PowerUp SYBR Green Master Mix (ThermoFisher), and normalized to actin as an internal control. Experiments were performed using a
StepOnePlus Real-Time PCR System (Applied Biosystems). poly(A)-mRNA was isolated using Ambion® Poly(A)Purist™ MAG Kit (ThermoFisher) according to manufacturer's protocol. Nuclear and cytoplasmic separation of RNA was performed with Thermo Scientific NE PER Nuclear and Cytoplasmic Extraction Kit (ThermoFisher) according to manufacturer's protocol. RNA in either the nuclear pellets or cytoplasmic fractions was extracted with RNeasy Plus kit (Qiagen). Total RNA extracted per fraction was quantified and used to calculate the percentage of mRNA localization for the RT-qPCR quantification. The list of primers is provided in Supplementary Data (Supplementary Table S1).

Tan Z., Fei G., Paulo J.A., Bellaousov S., Martin S.E., Duveau D.Y., Thomas C.J., Gygi S.P., Boutz P.L., & Walker S. (2020). O-GlcNAc regulates gene expression by controlling detained intron splicing.

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