Log In Sign up
The largest database of trusted experimental protocols

RNF125 is a ubiquitin-protein ligase that is involved in the regulation of cellular processes. It functions by catalyzing the attachment of ubiquitin to target proteins, thereby marking them for degradation or altering their activity.

Automatically generated - may contain errors

Lab products found in correlation

Rnf125, by Merck Group (2 mentions) P stat3, by Cell Signaling Technology (2 mentions) Hpa041514, by Merck Group (2 mentions) Tsa biotin plus system, by PerkinElmer (2 mentions)

2 protocols using rnf125

1

Immunohistochemical Analysis of RNF125 in Melanoma

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumor biopsies were stained with primary antibodies for RNF125 (HPA041514; Sigma-Aldrich) followed by a secondary antibody conjugated to horseradish peroxidase and treated with 3,3′-diaminobenzidine (DAB) chromogen. Stained slides were interpreted by a dedicated dermatopathologist. Only positive signals with clear melanoma morphology were evaluated. For immunofluorescence, the primary antibodies were RNF125 and pSTAT3 (both diluted 1:1,000; Cell Signaling), and signals were amplified using the TSA-Biotin Plus System (Perkin Elmer) according to the manufacturer’s instructions. The same slides were counterstained with S-100 (1:400; Dako). Immunofluorescence-stained slides were visualized using a fluorescence microscope with a Slidebook or Aperio slide scanner.
Kim H., Frederick D.T., Levesque M.P., Cooper Z.A., Feng Y., Krepler C., Brill L., Samuels Y., Hayward N.K., Perlina A., Piris A., Zhang T., Halaban R., Herlyn M.M., Brown K.M., Wargo J.A., Dummer R., Flaherty K.T, & Ronai Z.A. (2015). Downregulation of the Ubiquitin Ligase RNF125 Underlies Resistance of Melanoma Cells to BRAF Inhibitors via JAK1 Deregulation. Cell reports, 11(9), 1458-1473.
+ Open protocol
+ Expand
2

Immunohistochemical Analysis of RNF125 in Melanoma

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumor biopsies were stained with primary antibodies for RNF125 (HPA041514; Sigma-Aldrich) followed by a secondary antibody conjugated to horseradish peroxidase and treated with 3,3′-diaminobenzidine (DAB) chromogen. Stained slides were interpreted by a dedicated dermatopathologist. Only positive signals with clear melanoma morphology were evaluated. For immunofluorescence, the primary antibodies were RNF125 and pSTAT3 (both diluted 1:1,000; Cell Signaling), and signals were amplified using the TSA-Biotin Plus System (Perkin Elmer) according to the manufacturer’s instructions. The same slides were counterstained with S-100 (1:400; Dako). Immunofluorescence-stained slides were visualized using a fluorescence microscope with a Slidebook or Aperio slide scanner.
Kim H., Frederick D.T., Levesque M.P., Cooper Z.A., Feng Y., Krepler C., Brill L., Samuels Y., Hayward N.K., Perlina A., Piris A., Zhang T., Halaban R., Herlyn M.M., Brown K.M., Wargo J.A., Dummer R., Flaherty K.T, & Ronai Z.A. (2015). Downregulation of the Ubiquitin Ligase RNF125 Underlies Resistance of Melanoma Cells to BRAF Inhibitors via JAK1 Deregulation. Cell reports, 11(9), 1458-1473.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!