One day prior to being taken for experimentation, 10,000 U87MG-LTR cells stably expressing Tat-GFP were seeded for 24 h in 96-well plates. Cells were then treated for 6 or 24 h with vehicle or 25-μM concentrations of the iron chelators DFO, deferiprone, or 2–2 bipyridyl before being taken for determination of HIV-1 LTR activity using the Steady Glow Luciferase Assay (Promega); HIV-1 LTR activity was measured as relative luminescence units (RLU).
Steady glow luciferase assay
Manufactured by Promega
The Steady Glow Luciferase Assay is a luminescence-based detection system designed to measure luciferase reporter gene expression. The assay provides a stable luminescent signal, allowing for continuous monitoring of luciferase activity over extended periods of time.
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Lab products found in correlation
2 protocols using steady glow luciferase assay
1
Assessing HIV-1 LTR Activity with Iron Chelators
2
Generating Tat-GFP Expressing Cell Line
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