Erlotinib sml2156

Salt oleic acid (sodium oleate; sc-215879) was purchased from Santa Cruz (Santa Cruz). Linoleic acid sodium salt (L8134), sodium palmitate (P9767), N-acetylcysteine (NAC; A1965), U0126 (19-147), gefitinib (SML1657), and erlotinib (SML2156) were purchased from Sigma‒Aldrich (Sigma‒Aldrich, St Louis, MO, USA). Bovine serum albumin (fatty acid-free; A7030) (Sigma‒Aldrich, St Louis, MO, USA) was conjugated with oleic acid at a 1:8 molar ratio (BSA/OA). Carboxyl H2-DCFDA (D399) and EGF (PHG0311) were purchased from Invitrogen (Invitrogen, Grand Island, NY, USA). Recombinant human ANGPTL4 (rh-ANGPTL4: 4487-AN) was purchased from R&D Systems (R&D Systems, Minneapolis, MN). CXCL8 (ab9631) was purchased from Abcam (Abcam). Cetuximab was purchased from BioVision (A1047-100; BioVision). Vitamin E (25985), hygromycin B (hydrate: 14291), reparixin (21492), and atorvastatin (10493) were purchased from Cayman (Cayman). atorvastatin (Lipitor; VITARIS) was used for feeding mice.

The human osteosarcoma cell lines MG63 (ATCC^® CRL-1427^™) and U2OS (ATCC^® HTB-96^™) were obtained from American Type Culture Collection (Manassas, VA, USA), and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Sigma–Aldrich, St. Louis, MO, USA). All media were supplemented with 10% fetal bovine serum (FBS, Sigma–Aldrich). These cell lines were employed for the described experiments without further authentication. Sodium cantharidate (CN100506212C) was obtained from Shandong Luoxin Pharmaceutical Co., Ltd (Linyi, Shandong, China). LY5 (#562712) was purchased from MedKoo Biosciences, Inc. (Morrisville, NC, USA). Erlotinib (SML2156) was purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd (Shanghai, China). The lentivirus expressing a constitutively active mutant form of STAT3 (EF.STAT3C.Ubc.GFP) was a gift from Linzhao Cheng (Addgene plasmid #24983; http://n2t.net/addgene:24983; RRID: Addgene_24983) [53 (link)]. Vector pLenti-CMV-MCS-GFP-SV-puro was a gift from Paul Odgren (Addgene plasmid #73582; http://n2t.net/addgene:73582; RRID: Addgene_73582) [54 (link)]. Negative control siRNAs (sc-37007) and STAT-3-targeted siRNA (siSTAT3; sc-29493) were obtained from Santa Cruz Biotechnology, Inc. (Shanghai, China).