3200fs microscope

Manufactured by JEOL

The JEOL 3200FS is a high-resolution transmission electron microscope (TEM) designed for advanced materials analysis. It features a field emission gun (FEG) source, which provides high-brightness and high-resolution imaging capabilities. The microscope is equipped with advanced electron optics and a range of analytical tools, including energy-dispersive X-ray spectroscopy (EDS) and electron energy-loss spectroscopy (EELS).

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Lab products found in correlation

Vitrobot mark 4, by Thermo Fisher Scientific (3 mentions) K2 summit 4kx4k direct detector, by Ametek (2 mentions) 400 mesh copper grid, by Ted Pella (1 mentions) Ultrascan 4000, by Ametek (1 mentions) Whatman 1 filter paper, by Cytiva (1 mentions) Lacey carbon support film, by Ted Pella (1 mentions) Whatman, by Cytiva (1 mentions)

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JEM-3200FS microscope (2 citations)

4 protocols using 3200fs microscope

Cryo-EM Imaging of Nanoparticle Samples

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The various NP solutions
were vitrified and imaged at the Baylor College of Medicine Cryo-Electron
Microscopy Core Facility (BCM, Houston, TX). Quantifoil R2/1 + Cu
200 mesh holey carbon grids were pretreated with a 45 s air-glow discharge
to make the carbon surface hydrophilic. Alongside these grids, Quantifoil
R2/1 200Cu + 4 nm thin carbon grids were also glow discharged for
10 s to test the efficacy of the added layer of continuous carbon
with binding the NP. Vitrification was performed using a Vitrobot
Mark IV (FEI, Hillsboro, OR) operated at 18 °C and 100% humidity.
Each grid had 3 μL of NP sample applied to it and was blotted
for 1–3 s before being immediately submerged in liquid ethane.
The frozen grids were then transferred into a JEOL 3200FS microscope
(JEOL) outfitted with a Gatan K2 Summit 4k × 4k direct detector
(Gatan, Pleasanton, CA) and a postcolumn energy filter set to 30 eV.
Before imaging, the microscope was carefully aligned to prevent any
beam-induced aberrations or astigmatism that can negatively impact
image quality. Images were collected at magnifications of 15,000×
and 30,000× with respective pixel sizes of 2.392 and 1.232 Å.
Images were collected using an exposure time of 1 s with an approximate
dose rate of ∼20e^–/Å²/s per
image.

Zinger A., Sushnitha M., Naoi T., Baudo G., De Rosa E., Chang J., Tasciotti E, & Taraballi F. (2021). Enhancing Inflammation Targeting Using Tunable Leukocyte-Based Biomimetic Nanoparticles. ACS Nano, 15(4), 6326-6339.

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Vitrification and Cryo-EM Imaging of Nanoparticles

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NPs were imaged at the Baylor College of Medicine Cryo-Electron Microscopy Core Facility (Houston, TX). Quantifoil R2/1, Cu 200 mesh Holey Carbon grids were pretreated with a 45-s air-glow discharge to make the carbon surface hydrophilic. The use of a Vitrobot Mark IV (FEI, Hillsboro, OR) operated at 18 °C and 100% humidity allowed vitrification of NPs to occur. 3 μl of NP sample was loaded into each grid and blotted for 1–3 s before immersion in liquid ethane. Grids were imaged with a JEOL 3200FS microscope (JEOL) using a Gatan K2 Summit 4kx4k direct detector (Gatan, Pleasanton, CA) and a post-column energy filter set to 30 eV. Images were acquired using a 1-s exposure time.

Mancino C., Pasto A., De Rosa E., Dolcetti L., Rasponi M., McCulloch P, & Taraballi F. (2023). Immunomodulatory biomimetic nanoparticles target articular cartilage trauma after systemic administration. Heliyon, 9(6), e16640.

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Transmission Electron Microscopy Sample Preparation

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Each sample was applied to an ultra-thin carbon film over Lacey Carbon Support Film on 400-mesh copper grids (Ted Pella, Inc.) that were glow discharged for 1 minute. Excess solution was blotted with Whatman #1 filter paper and the grid was rapidly stained with 2% uranyl acetate. The uranyl acetate was immediately blotted with Whatman #1 filter paper and rapidly stained with 2% methylamine tungstate. The second stain was immediately blotted away with Whatman #1 filter paper and allowed to air dry. Data was collected on a JEOL 3200FS microscope operated at 300 kV. Images were acquired at a magnification of 46,000x and at 1.0 μm underfocus using a Gatan UltraScan 4000 charge-coupled device (CCD) camera.

Calton C.M., Bronnimann M.P., Manson A.R., Li S., Chapman J.A., Suarez-Berumen M., Williamson T.R., Molugu S.K., Bernal R.A, & Campos S.K. (2017). Translocation of the papillomavirus L2/vDNA complex across the limiting membrane requires the onset of mitosis. PLoS Pathogens, 13(5), e1006200.

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Cryo-EM Characterization of Liposomes, Leukosomes and NPs

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The morphology of liposomes and leukosomes and NPs solutions were determined and imaged at the Baylor College of Medicine Cryo‐Electron Microscopy Core Facility (BCM, Houston, TX). The Quantifoil R2/1, Cu 200 mesh Holey Carbon grids were pretreated with a 45 s air‐glow discharge to make the carbon surface hydrophilic. Alongside these grids, Quantifoil R2/1 200Cu +4 nm thin carbon grids were also glow discharged for 10 s to test the efficacy of the added layer of continuous carbon with binding of the NPs. Vitrification was performed using a Vitrobot Mark IV (FEI, Hillsboro, OR) operated at 18 °C and 100% humidity. Each grid had 3 µL of NPs sample applied to it and was subsequently blotted for 1–3 s before being immediately submerged in liquid ethane. The frozen grids were then transferred into a JEOL 3200FS microscope (JEOL) outfitted with a Gatan K2 Summit 4kx4k direct detector (Gatan, Pleasanton, CA) and a postcolumn energy filter set to 30 eV. Before imaging, the microscope was carefully aligned to prevent any beam‐induced aberrations or astigmatism that can negatively impact image quality. Images were collected at magnifications of 15 000× and 30 000× with respective pixel sizes of 2.392 and 1.232 angstroms. Images were collected using an exposure time of 1 s with an approximate dose rate of ≈20e‐/Å²/s per image.

Zinger A., Soriano S., Baudo G., De Rosa E., Taraballi F, & Villapol S. (2021). Biomimetic Nanoparticles as a Theranostic Tool for Traumatic Brain Injury. Advanced Functional Materials, 31(30), 2100722.

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