The immunohistochemistry procedures were carried out according to the previous method with appropriate modifications [27 (link)]. The tissue sections were incubated with PTEN antibody (ab170941, 1:100) and stained with horseradish peroxidase (HRP) anti-rabbit immunoglobulin G and diaminobenzidine. Next, the sections were incubated with secondary antibody and stained with EnVision G2 System/AP Rabbit Mouse (Permanent Red) (Dako, Glostrup, Denmark). Lastly, the sections were counterstained with hematoxylin and observed under a fluorescence inverted microscope (Hitachi Limited, Tokyo, Japan).
Fluorescence inverted microscope
Manufactured by Hitachi
Sourced in Japan
The Fluorescence inverted microscope is a specialized laboratory instrument designed for high-resolution imaging of fluorescently labeled samples. It features an inverted optical design, where the light source and objectives are positioned below the specimen, allowing for efficient detection of emitted fluorescence signals.
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Lab products found in correlation
2 protocols using fluorescence inverted microscope
1
Immunohistochemical Detection of PTEN
2
CREBBP Immunohistochemical Staining
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The prepared tumor tissue sections were incubated with CREBBP antibody (1 : 1,000, ab2832) and stained with HRP-labeled anti-rabbit IgG or diaminobenzidine. Moreover, the sections were incubated with a mouse secondary antibody and stained with EnVision G2 System/AP Rabbit Mouse Permanent Red (Dako, Glostrup, Denmark). Finally, the sections were counterstained with hematoxylin and observed under a fluorescence inverted microscope (Hitachi Limited, Tokyo, Japan).
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