Dacinostat

We used an "epigenetics compound library" from Selleckchem, containing 181 compounds (Supplementary Table 6), dissolved in DMSO or water (172 and 9 compounds respectively). NPCs were plated at a density of 7.5x10 4 cells/cm 2 in 96-well plates coated with gelatin 0.1%. Six hours later, compounds were added to each well at a final concentration of 10 µM and 0.5% of solvent (DMSO or water). Cells were collected 48 h later for lysis and reverse transcription was performed using the Cells-to-Ct kit (Applied Biosystems) directly in 96-well plates. The allelic expression ratio of Bag3 was then measured using pyrosequencing after PCR. The 11 best hits were then tested following the same protocol, in 24-well plates. The dose-response curves were performed in 6-well plates, with various concentrations of Dacinostat and Decitabine and a constant concentration of DMSO (0.5%). The final validation was performed in a 6-well plate format, using new batches of Dacinostat and Decitabine (Selleck Chemicals), dissolved in DMSO; cells were treated for 24 h with 10 µM of drugs, in 0.5% DMSO. For assays in 24-well and 6-well plates, cells were harvested and RNAs extracted using the Qiagen RNeasy plus mini kit, as described earlier.