A total of 7902 compounds from drug libraries including StemSelect (EMD), Spectrum (Microsources), LOPAC (Sigma), FDA/IN drugs (Microsources), Prestwick chem library (Prestwick), LeadGen collection (Enzo Life Science), InhibitorSelect Pathway (EMD), NIH clinical collection (NIH), NCI oncology drugs (NCI), Epigenetics library (Enzo Life Science), Kinase inhibitors (EMD), Kinase inhibitors (Cayman), Kinase inhibitors (SeleckChem, Drug Reposition Library (various vendors). Compounds were loaded into 384 well plates at a concentration of 1 μM and 5000 CPC cells were seeded into each well. Celltiter Glo assay was used to determine the effect of drugs on cell viability 48 hours later. More than 50% reduction in viability compared to vehicle control (DMSO) treated cells was used as a cut off to select candidate compounds.
Epigenetics library
Manufactured by Enzo Life Sciences
Sourced in United Kingdom
The Epigenetics library is a comprehensive collection of products designed for epigenetics research. It includes a variety of reagents and tools for the study of DNA methylation, histone modifications, and other epigenetic mechanisms.
Automatically generated - may contain errors
2 protocols using epigenetics library
1
Screening Compounds for Cell Viability
2
High-Throughput Drug Screening of Patient-Derived Cells
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Cells were seeded semi-automatically in 384-well plates (Corning) using a Multidrop dispenser (Thermo Fisher Scientific) Patient-derived cells were seeded at a concentration of 1–1.5 × 106 cells/ml, the seeding density of the cell lines are provided in Supplemental Table 2. On the same day, drugs were added to a final concentration of 10 nM, 100 nM or 1000 nM, using the Caliper SciClone ALH3000 liquid handling robot. The following commercially available drug libraries were utilized: Prestwick Chemical library (Prestwick Chemical, France), anti-neoplastic sequoia library (Sequoia Research Products, United Kingdom), Epigenetics library (Enzo Life Sciences, Belgium), Epigenetics screening library (Cayman Chemical, MI, USA), Spectrum collection (Microsource, CT, USA), the Cell cycle/DNA Damage compound library (MedChemExpress, Sweden), and some additional compounds (Supplemental Table 3). Upon drug exposure, cell viability was assessed by a 4-day thiazolyl blue tetrazolium bromide (MTT; Sigma-Aldrich) assay [13] (link).
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