2 aminoethoxydiphenylborane 2 apb

GLY and TIO were purchased from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in phosphate-buffered saline (PBS). GLY was used at 300µM for the intranasal administration to mice and at 100 nM for the organ culture of murine tracheal tissue and primary NHBE cell culture under air–liquid interface (ALI) conditions. TIO was used at 100 nM for culture conditions. 2-Aminoethoxydiphenylborane (2-APB), an inhibitor of inositol-trisphosphate receptor, was purchased from Tocris Bioscience (Bristol, UK) and used at 40 μM. N-[2-(p-bromocinnamylamino) ethyl]-5-isoquinolinesulfonamide, dihydrochloride (H89), a protein kinase inhibitor with a high specificity for protein kinase A (PKA), was purchased from Cayman Chemical (Ann Arbor, MI, USA) and used at 10 μM. Telenzepine, M1 receptor antagonist, was purchased from Tocris Bioscience (Bristol, UK) and used at 10 μM. Gallamine, M2 receptor antagonist, was purchased from Sgma-Aldrich and used at 30 μM. 4-DAMP, M3 receptor antagonist, was purchased from Abcam (Cambridge, UK) and used at 10 μM. Thapsigargin were purchased from Sigma-Aldrich and used at 1 μM.