Log In Sign up
The largest database of trusted experimental protocols

Compatible guard column

Manufactured by Merck Group
Visit Supplier

The Compatible Guard Column is a laboratory equipment designed to protect the analytical column by filtering out particulates and contaminants from the sample. It is placed before the analytical column to prevent premature deterioration and ensure the reliable performance of the analytical system.

Automatically generated - may contain errors

Lab products found in correlation

Vanquish uplc system, by Thermo Fisher Scientific (2 mentions) Compound discoverer, by Thermo Fisher Scientific (2 mentions) Xcalibur, by Thermo Fisher Scientific (2 mentions) Q exactive plus orbitrap mass spectrometer, by Thermo Fisher Scientific (2 mentions) Discovery hs f5 hplc column, by Merck Group (2 mentions) Tracefinder, by Thermo Fisher Scientific (2 mentions)

2 protocols using compatible guard column

1

Metabolite Extraction and Quantification

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The cells were subjected to metabolite extraction using our established protocol as described before.59 (link)–61 (link) The lyophilized aqueous phase metabolite samples were re-suspended in 50 % (vol/vol) acetonitrile diluted with mass-spec-grade water, while the dried organic phase metabolite samples were re-suspended in 2: 1 (vol: vol) of chloroform: methanol. Metabolomics data from the samples were acquired using a Thermo Scientific Q Exactive Orbitrap Mass Spectrometer Plus with a Vanquish UPLC system at Johns Hopkins Metabolomics Facility overseen by Dr. Anne Le. The Vanquish UPLC auto-sampler systems were used to uptake 2 μl of each sample and were maintained at 4 °C. Reverse-phase chromatography using 0.1 % formic acid in mass spec-grade water as mobile aqueous phase and 0.1 % formic acid in 98 % acetonitrile as the mobile organic phase was employed. The total runtime for each sample was 13 minutes. A Discovery® HS F5 HPLC Column with 3 μm particle size and 15 cm × 2.1 mm L × I.D. (Sigma) and a compatible guard column (Sigma) were used and were maintained at 35 °C. Data were analyzed using Thermo Fisher Scientific Compound discoverer®, Xcalibur®, and TraceFinder® softwares. The raw intensities were normalized based on protein concentration and cell weight of each sample to get the final normalized intensities.
Cui L., Gouw A.M., LaGory E.L., Guo S., Attarwala N., Tang Y., Qi J., Chen Y.S., Gao Z., Casey K.M., Bazhin A.A., Chen M., Hu L., Xie J., Fang M., Zhang C., Zhu Q., Wang Z., Giaccia A.J., Gambhir S.S., Zhu W., Felsher D.W., Pegram M.D., Goun E.A., Le A, & Rao J. (2020). ED SUM: Triple-negative breast cancer is inhibited by depleting mitochondrial copper in mice.: Mitochondrial copper depletion suppresses triple-negative breast cancer in mice. Nature biotechnology, 39(3), 357-367.
+ Open protocol
+ Expand
2

Metabolite Extraction and Quantification

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The cells were subjected to metabolite extraction using our established protocol as described before.59 (link)–61 (link) The lyophilized aqueous phase metabolite samples were re-suspended in 50 % (vol/vol) acetonitrile diluted with mass-spec-grade water, while the dried organic phase metabolite samples were re-suspended in 2: 1 (vol: vol) of chloroform: methanol. Metabolomics data from the samples were acquired using a Thermo Scientific Q Exactive Orbitrap Mass Spectrometer Plus with a Vanquish UPLC system at Johns Hopkins Metabolomics Facility overseen by Dr. Anne Le. The Vanquish UPLC auto-sampler systems were used to uptake 2 μl of each sample and were maintained at 4 °C. Reverse-phase chromatography using 0.1 % formic acid in mass spec-grade water as mobile aqueous phase and 0.1 % formic acid in 98 % acetonitrile as the mobile organic phase was employed. The total runtime for each sample was 13 minutes. A Discovery® HS F5 HPLC Column with 3 μm particle size and 15 cm × 2.1 mm L × I.D. (Sigma) and a compatible guard column (Sigma) were used and were maintained at 35 °C. Data were analyzed using Thermo Fisher Scientific Compound discoverer®, Xcalibur®, and TraceFinder® softwares. The raw intensities were normalized based on protein concentration and cell weight of each sample to get the final normalized intensities.
Cui L., Gouw A.M., LaGory E.L., Guo S., Attarwala N., Tang Y., Qi J., Chen Y.S., Gao Z., Casey K.M., Bazhin A.A., Chen M., Hu L., Xie J., Fang M., Zhang C., Zhu Q., Wang Z., Giaccia A.J., Gambhir S.S., Zhu W., Felsher D.W., Pegram M.D., Goun E.A., Le A, & Rao J. (2020). ED SUM: Triple-negative breast cancer is inhibited by depleting mitochondrial copper in mice.: Mitochondrial copper depletion suppresses triple-negative breast cancer in mice. Nature biotechnology, 39(3), 357-367.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!