Cells cultured in adipocyte differentiation medium (dexamethasone 2.0 μM, insulin 20 μg/mL, indomethacin 400 μM, and IBMX 1.0 mM) were washed with PBS and then fixed in 4% paraformaldehyde for 10 minutes. Lipid substance was stained with Oil-Red following the manufacturer’s instruction (Solarbio Co., Beijing, China).
Oil red
Manufactured by Solarbio
Sourced in China
Oil red is a fat-soluble dye used for staining neutral lipids and triglycerides in cells and tissues. It is commonly used in histological and cytological applications to visualize and identify lipid-rich structures.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using oil red
1
Adipocyte Differentiation and Lipid Staining
2
Oil-red Staining of VSMCs
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VSMCs were fixed in 4% PFA in phosphate-buffered saline (PBS) and then rinsed with PBS three times. The cells were then stained with Oil-red (Solarbio, Beijing) for 30 min at room temperature. Staining was assessed by optical microscopy.
3
Adipogenic Differentiation and Muscular Fatty Infiltration
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Tissue slices or cultured cells were first fixed in 4% paraformaldehyde for 10 min, followed by oil red (Solarbio, cat#G1260) treatment for 10 min. Then samples were stained with hematoxylin for 5 min and mounted with 10% glycerol. For quantification analysis of lipid after adipogenic differentiation, the oil red was extracted by isopropanol for 5 min, and the value at 496 nm absorbance was detected by a microplate reader (Thermo Fisher Scientific).
Triglycerides colorimetric assay kit (Elabscience, cat#E-BC-K261-M) was employed to quantify the muscular fatty infiltration after tendon injury. Briefly, the muscle was first grounded with isopropanol (ACMEC, cat#I86620). Then tissue fragments were discarded by centrifugal machine at 10000 × g for 3 min. The supernatant was used to detect triglycerides by microplate reader at 510 nm absorbance.
Triglycerides colorimetric assay kit (Elabscience, cat#E-BC-K261-M) was employed to quantify the muscular fatty infiltration after tendon injury. Briefly, the muscle was first grounded with isopropanol (ACMEC, cat#I86620). Then tissue fragments were discarded by centrifugal machine at 10000 × g for 3 min. The supernatant was used to detect triglycerides by microplate reader at 510 nm absorbance.
4
Assessing ASC Proliferation and Differentiation
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The methods used to assay ASC proliferation and differentiation have been previously described.11 ASC proliferation was assayed with a methylthiazol tetrazolium (MTT) method and colony formation. ASC osteogenic and adipogenic differentiation were induced by culture in adipogenic and osteogenic media. Oil red (Solarbio, G1262) and alizarin (Solarbio, G8550 red were used to detect the formation of lipid droplets and calcium.
5
Histological Analysis of Mouse Aortic Roots
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Mouse aortic roots were sequentially sliced and subsequently stained with HE (Solarbio, China), Masson (Solarbio, China) and Oil red (Solarbio, China) according to the manufacturer's instructions.
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