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Rabbit anti py397

Manufactured by Thermo Fisher Scientific
Sourced in United States

Rabbit anti-pY397 is an antibody that specifically recognizes the phosphorylated tyrosine 397 residue. This antibody can be used for detection and quantification of the phosphorylated form of the target protein in various applications such as Western blotting, immunohistochemistry, and ELISA.

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2 protocols using rabbit anti py397

1

Biochemical Analysis of FAK and Src Signaling

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Recombinant human leptin, FAK (PF-573228) and Src (PP2) inhibitors were obtained from Sigma-Aldrich (St Louis, MO, USA). Mouse anti-actin, rabbit anti-FAK and anti-Src antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit anti-GAPDH antibody was from Cell Signaling Technologies (Danvers, MA, USA). The phospho-specific antibody against FAK, rabbit anti-pY397, was obtained from Invitrogen (Carlsbad, CA, USA). The phospho-specific antibody against Src, rabbit anti-pY418, was obtained from MyBiosource (San Diego, CA, USA). The secondary HRP-conjugated antibodies were from Millipore (Billerica, MA, USA), and the anti-mouse and anti-rabbit conjugated with Alexa Fluor 488 antibodies were from Invitrogen (Carlsbad, CA, USA). TRITC conjugated phalloidin was purchased from Cytoskeleton (Denver, CO, USA).
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2

Inhibition of FAK, Src, and STAT3 Signaling

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Recombinant human leptin, FAK (PF-573228) and Src (PP2) inhibitors were obtained from Sigma-Aldrich. The Src (SU6656) and the STAT3 inhibitor (S31201) were from Merck. Mouse anti-actin, rabbit anti-FAK and anti-Src antibodies were purchased from Santa Cruz Biotechnology. The phospho-specific antibody against FAK, rabbit anti-pY397, was obtained from Invitrogen. The phospho-specific antibody against Src, rabbit anti-pY418, was obtained from MyBiosource (San Diego, CA, USA), species-specific secondary HRP-conjugated antibodies were from Millipore and the secondary antibody anti-rabbit conjugated with Alexa Fluor 488 was from Invitrogen. Phalloidin coupled to TRITC was purchased from Cytoskeleton (Denver, CO, USA).
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