Prolong mounting medium with dapi

Manufactured by Thermo Fisher Scientific

Prolong Mounting Medium with DAPI is a laboratory reagent designed for use in fluorescence microscopy. It is a aqueous-based mounting medium that helps preserve fluorescent signals and provides nuclear counterstaining with DAPI dye.

Automatically generated - may contain errors

Lab products found in correlation

Mitotracker deep red, by Thermo Fisher Scientific (1 mentions) Methocult, by STEMCELL (1 mentions) Z2 microscope, by Zeiss (1 mentions) Xylene, by Thermo Fisher Scientific (1 mentions) Thunder microscope, by Leica (1 mentions) C2 confocal microscope, by Nikon (1 mentions) C2 plus confocal microscope, by Nikon (1 mentions) Fibronectin coated coverslips, by Merck Group (1 mentions) Eclipse ti e inverted microscope system, by Nikon (1 mentions)

5 protocols using prolong mounting medium with dapi

Mitochondrial Dynamics and Autophagy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sorted LT and ST cells were cultured in complete growth medium in presence or absence of ± 500mM NR for 24hours and treated with 5nM Bafilomycin or PBS for additional 12 h. The cells were treated with Mitotracker Deep Red (Thermo Scientific), fixed with PFA 4%, stained for Lc3b (Cell Signaling) and mounted using Prolong mounting medium with DAPI (Thermo Scientific). For Lc3b, we used donkey anti-rabbit 555 secondary antibody (Alexa). The fluorescent pictures were taken using Zeiss 880LSM Airyscan. 3D whole cell reconstructions were analyzed using Imaris software (Bitplane) to calculate Lc3b punctae number and mitochondria surface, utilizing dot and surface analyses functions.
CFUs assay 12500 total bone marrow cells were isolated from the legs and hips of C57BL/6J mice and cultured in MethoCult (StemCell technology) containing recombinant SCF, EPO, IL-3 and IL-6. Number and morphology of the colonies were analyzed with phase contrast microscope after 7 days of culture.

Vannini N., Campos V., Girotra M., Trachsel V., Rojas-Sutterlin S., Tratwal J., Ragusa S., Stefanidis E., Ryu D., Rainer P.Y., Nikitin G., Giger S., Li T.Y., Semilietof A., Oggier A., Yersin Y., Tauzin L., Pirinen E., Cheng W.C., Ratajczak J., Canto C., Ehrbar M., Sizzano F., Petrova T.V., Vanhecke D., Zhang L., Romero P., Nahimana A., Cherix S., Duchosal M.A., Ho P.C., Deplancke B., Coukos G., Auwerx J., Lutolf M.P, & Naveiras O. (2019). The NAD-Booster Nicotinamide Riboside Potently Stimulates Hematopoiesis through Increased Mitochondrial Clearance. Cell stem cell, 24(3).

+ Open protocol

+ Expand

Immunofluorescence Labeling of Adherent Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells growing on glass coverslips were fixated with 4% (v/v) PFA (paraformaldehyde) and 4% (w/v) sucrose solution (20 minutes at RT), permeabilized with 0.3% (v/v) Triton X-100 solution (15 minutes, RT) and then blocked with BSA 1% (w/v) and Triton X-100 0.1% (w/v) for half hour at RT. Later, cells were probed with primary (2h incubation) and secondary (1h) antibodies, as described in Supplementary Table 1. Coverslips were mounted onto glass slides with Prolong mounting medium (with DAPI 1:1000 (4',6-diamidino-2-phenylindole, Thermo-Fisher Scientific). Washing with ice-cold PBS was always performed between steps. All antibodies used were diluted in 1%(v/v) BSA and 0,1%(v/v) Triton X-100 solution. Fixating, permeabilizing and blocking solutions were prepared in PBS. Images were captured with a Zeiss Z2 microscope, with at least 5 random micrographs being acquired, unless otherwise stated.

Soares N.L., Paiva I., Bravo J., Queiroga C.S.F., Melo B.F., Conde S.V., Romão C.C., Summavielle T., & Vieira H.L.A. (2021). Carbon Monoxide Modulation of Microglia-Neuron Communication: Anti-Neuroinflammatory and Neurotrophic Role.

+ Open protocol

+ Expand

Adult and Embryonic Brain Immunostaining

Check if the same lab product or an alternative is used in the 5 most similar protocols

For adult brain immunostaining, paraffin blocks were sliced into 3 µm sections and incubated at 56°C overnight. The next day, tissue sections were deparaffinized with xylene (Fisher Scientific, Waltham, MA, United States) and rehydrated with decreasing concentrations of ethanol in water. Antigen retrieval was performed with boiling sodium citrate buffer at pH 6 for 7 min and sections were permeabilized with 1% Tween in PBS for 15 min. Embryonic brain cryosections were permeabilized with 0.5% Triton X-100 for 30 min. Adult and embryonic brain sections were blocked with PBS, 0.2% BSA, 5% goat serum, 0.2% glycine and 0.2% lysine for 2 hours at room temperature. Primary antibodies were diluted in PBS, 0.3% Triton X-100 and 2% goat serum and secondary antibodies in PBS, 0.3% Triton X-100 and 3% goat serum. Sections were mounted in Prolong Mounting Medium with DAPI (Invitrogen). Immunofluorescence images were taken with a C2 confocal microscope (Nikon) at ×20 magnification and applying optimal digital zoom calculated with the Nyquist-Shannon sampling theorem. Overviews of the adult and mouse brain were taken with a Leica Thunder microscope.

Senís E., Esgleas M., Najas S., Jiménez-Sábado V., Bertani C., Giménez-Alejandre M., Escriche A., Ruiz-Orera J., Hergueta-Redondo M., Jiménez M., Giralt A., Nuciforo P., Albà M.M., Peinado H., del Toro D., Hove-Madsen L., Götz M, & Abad M. (2021). TUNAR lncRNA Encodes a Microprotein that Regulates Neural Differentiation and Neurite Formation by Modulating Calcium Dynamics. Frontiers in Cell and Developmental Biology, 9, 747667.

+ Open protocol

+ Expand

Immunofluorescence Staining of NIH3T3 and N2A Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

NIH3T3 and N2A cells were fixed in 4% paraformaldehyde for 15 min and permeabilized with 0.5% Triton X-100 for 7 min at room temperature. Cells were blocked in 3% Bovine Serum Albumin (BSA) and 10% goat serum in PBS for 1 hour and incubated overnight at 4°C with the primary antibody diluted in 3% BSA in PBS. Next day, secondary antibodies were incubated for 1 hour at room temperature in the dark. Cells were mounted in Prolong Mounting Medium with DAPI (Invitrogen). Images were taken in a Nikon C2 Plus Confocal Microscope.

+ Open protocol

+ Expand

Immunofluorescence Staining of Adherent Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded in fibronectin-coated coverslips (Sigma Aldrich). When desired, cells were fixed in 2% paraformaldehyde for 15 min and permeabilized with 0.5% Triton X-100 for 10 min at room temperature. Blocking step was made in 3% Bovine Serum Albumin (BSA) for 1 h. Cells were incubated overnight at 4 °C with the primary antibody diluted in blocking buffer. Next day, secondary antibodies were incubated for 1 h at room temperature in the dark. Antibodies and dilutions are listed in Supplementary Table 7. Finally, cells were mounted in Prolong Mounting Medium with DAPI (Invitrogen) and images were taken in a Nikon Eclipse Ti-E inverted microscope system. For quantification, at least 200 cells per staining were evaluated using ImageJ software.

Boix O., Martinez M., Vidal S., Giménez-Alejandre M., Palenzuela L., Lorenzo-Sanz L., Quevedo L., Moscoso O., Ruiz-Orera J., Ximénez-Embún P., Ciriaco N., Nuciforo P., Stephan-Otto Attolini C., Albà M.M., Muñoz J., Tian T.V., Varela I., Vivancos A., Ramón y Cajal S., Muñoz P., Rivas C, & Abad M. (2022). pTINCR microprotein promotes epithelial differentiation and suppresses tumor growth through CDC42 SUMOylation and activation. Nature Communications, 13, 6840.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!