Micromist borosilicate glass nebulizer

Elemental quantification of purified Ruc with and without NEM and a buffer control was performed using an Agilent 7700 inductively coupled plasma mass spectrometer (Agilent, Santa Clara, CA) attached to a Teledyne Cetac Technologies ASX-560 autosampler (Teledyne Cetac Technologies, Omaha, NE). The following settings were fixed for the analysis: cell entrance, −40 V; cell exit, −60 V; plate bias, −60 V; OctP bias, −18 V; and collision cell helium flow, 4.5 ml/min. Optimal voltages for extract 2, omega bias, omega lens, OctP RF, and deflect were determined empirically before each sample set was analyzed. Element calibration curves were generated using Aristar ICP standard mix (VWR, Radnor, PA). Samples were introduced by peristaltic pump with 0.5-mm internal diameter tubing through a MicroMist borosilicate glass nebulizer (Agilent). Samples were initially taken at 0.5 revolutions per second (rps) for 30 s followed by 30 s at 0.1 rps to stabilize the signal. Samples were analyzed in spectrum mode at 0.1 rps, collecting three points across each peak and performing three replicates of 100 sweeps for each element analyzed. Sampling probe and tubing were rinsed for 20 s at 0.5 rps with 2% nitric acid between every sample. Data were acquired and analyzed using the Agilent Mass Hunter workstation software version A.01.02.

Elemental quantification on acid-digested liquid samples was performed using an Agilent 7700 inductively coupled plasma mass spectrometer (Agilent, Santa Clara, CA) attached to a Teledyne CETAC Technologies ASX-560 autosampler (Teledyne CETAC Technologies, Omaha, NE). The following settings were fixed for the analysis: Cell Entrance = −40 V; Cell Exit = −60 V; Plate Bias = −60 V; OctP Bias = −18 V; and collision cell Helium Flow = 4.5 ml/min. Optimal voltages for Extract 2, Omega Bias, Omega Lens, OctP RF, and Deflect were determined empirically before each sample set was analyzed. Element calibration curves were generated using ARISTAR ICP Standard Mix (VWR, Radnor, PA). Samples were introduced by peristaltic pump with 0.5 mm internal diameter tubing through a MicroMist borosilicate glass nebulizer (Agilent). Samples were initially taken up at 0.5 rps for 30 s followed by 30 s at 0.1 rps to stabilize the signal. Samples were analyzed in Spectrum mode at 0.1 rps, collecting three points across each peak and performing three replicates of 100 sweeps for each element analyzed. The sampling probe and tubing were rinsed for 20 s at 0.5 rps with 2% nitric acid between every sample. Data were acquired and analyzed using the Agilent Mass Hunter Workstation Software version A.01.02. Data of the investigated metal ion were normalized to the ³⁴S abundance of each sample.