Rhodamine pam3csk4

Manufactured by InvivoGen

Sourced in United States

Rhodamine-Pam3CSK4 is a synthetic, fluorescently-labeled ligand that specifically binds to and activates Toll-like receptor 2 (TLR2). It is composed of the TLR2 agonist Pam3CSK4 conjugated to the rhodamine fluorescent dye.

Automatically generated - may contain errors

Lab products found in correlation

Resomer rg 503h, by Merck Group (1 mentions) Lipopolysaccharide lps, by Merck Group (1 mentions) Fitc lps, by Merck Group (1 mentions) Quant it oligreen ssdna reagent, by Thermo Fisher Scientific (1 mentions) Pam3csk4, by InvivoGen (1 mentions) Lsm 880, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions) Airyscan, by Zeiss (1 mentions)

Close spelling variants of this product

Pam3CSK4 (641 citations)

2 protocols using rhodamine pam3csk4

Lipid-Mediated Gene Delivery Optimization

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Poly(D,L-lactide-co-glycolide) (Resomer^® RG 503H, free carboxylic acid, MW 24–38 kD), dichloromethane, polyvinyl alcohol (PVA, MW 30–70 kD, 87–90% hydrolyzed), polyethylenimine (linear, MW 2.5 kD), poloxamer 407, class B CpG ODN 2007 (phosphorothioate backbone modified with a sequence of 5'-TCGTCGTTGTCGTTTTGTCGTT-3' and its non-CpG ODN form, FITC-LPS (derived from Escherichia coli O111:B4) and lipopolysaccharide (LPS) (derived from Escherichia coli O111:B4, TLR ligand tested) were obtained from Sigma-Aldrich (Oakville, ON, Canada). Pam3CSK4 and Rhodamine-Pam3CSK4 (endotoxin level tested) were obtained from InvivoGen (San Diego, CA, USA). Quant-iT™ OliGreen^® ssDNA reagent was from Life Technologies.

Alkie T.N., Taha-Abdelaziz K., Barjesteh N., Bavananthasivam J., Hodgins D.C, & Sharif S. (2017). Characterization of Innate Responses Induced by PLGA Encapsulated- and Soluble TLR Ligands In Vitro and In Vivo in Chickens. PLoS ONE, 12(1), e0169154.

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Rhodamine-Pam3CSK4 Visualization in TLR2-Expressing Cells

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HEK-hTLR2 cells (3 × 10⁴ cells per well) were seeded in 96-well plate (Cellvis, P96-1-N) with 100 μL DMEM medium (with 10% FBS and 1% pen/strep) and incubated at 37 °C overnight. The supernatant was removed and 50 μL fresh DMEM medium which contained 800 ng/mL Rhodamine-Pam₃CSK₄ (InvivoGen, tlr-rpms) and 1 μmol/L SMU-Y6 was added in for 4 h incubation. The supernatant was removed and the cells were washed with PBS for three times. DAPI (Beyotime, C1005) was added in and incubated for 5 min. Later, The DAPI was removed and the cells were washed with PBS for three times. The cells were observed under confocal microscopy (Carl Zeiss, LSM 880 with Airyscan).

Yang J., Pan Y., Zeng X., Liu S., Chen Z, & Cheng K. (2023). Discovery of novel aporphine alkaloid derivative as potent TLR2 antagonist reversing macrophage polarization and neutrophil infiltration against acute inflammation. Acta Pharmaceutica Sinica. B, 13(9), 3782-3801.

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