Dual throat swabs were taken simultaneously from all patients with clinical pharyngitis using rayon-tipped swabs. The first swab was taken to the laboratory, streaked onto a 5% sheep blood agar dish and incubated in an atmosphere of 5% CO2 at 35°C for 24–48 hours. After overnight incubation, the plate was examined to detect the presence of beta-haemolytic colonies. GAS was identified using the bioMérieux Vitek MS V.2.0 system (bioMérieux, France) according to the manufacturer’s instructions.
Vitek ms v 2.0 system
Manufactured by bioMérieux
Sourced in France
The Vitek MS V.2.0 system is a mass spectrometry-based identification instrument designed for microbial identification in clinical laboratories. It utilizes matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) technology to rapidly and accurately identify a wide range of microorganisms, including bacteria and yeasts, from cultured isolates.
Automatically generated - may contain errors
3 protocols using vitek ms v 2.0 system
1
Rapid Detection of Group A Streptococcus
2
Robust Pathogen Identification Protocol
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After sixty-eight hours of incubation, the blood culture (aerobic bottle) in a BACTEC 9000 system (Becton Dickinson, Sparks, MD) was positive and pathogen identification was carried out immediately. Briefly, approximately 1 ml of the positive blood culture was used to inoculate Columbia Blood agar, Chocolate agar and China Blue Lactose agar (Oxoid, Wesel, Germany) respectively, which were then incubated at 35±1°C with 5% CO2 supplement. Staining tests (Gram staining plus Acid fast staining or not), automated identification systems (Vitek 2 compact system, bioMérieux, France; BD phoenix 100 automated microbiology system, Becton, USA) and MALDI-TOF MS systems (Vitek MS v.2.0 system, bioMérieux, France; Bruker Biotyper v.3.1 system with the available database DB 5989, Bruker Daltonics, Germany) were employed to identify the positive colonies grew on the agar plates. 16S rRNA sequencing analysis with the universal primers of 27F and 1492R was performed for a precise species-level identification [5 (link), 6 ]. No other specimens were sent for microbiological examination.
3
Prevalence of E. coli Mastitis in Dairy Cows
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A total of 374 E. coli isolates from dairy mastitic milk were collected for this study, including 169 from the Animal and Plant Quarantine Agency (QIA; 76, 46, 16, and 31 isolates in 2012 , 2013 , 2014 , and 2015, respectively) and 205 from 16 local veterinary service agencies (78, 67, and 60 isolates in 2012, 2013, and 2014, respectively) . A total of 4,437 quarter milk samples from lactating cows from 137 dairy herds nationwide were collected in the Mastitis Diagnostic Laboratory of the QIA from 2012 to 2015. The proportion of samples having somatic cell counts of greater than 200,000 cells/mL was 39.3% (1,742/4,437 samples). However, no information on the numbers of samples, cattle, and farms was available from 16 local veterinary service agencies. Sixteen local veterinary service laboratories submitted data to the QIA on the E. coli isolates each year. The laboratories are located in every province. Quarter samples were obtained from cattle affected by clinical and subclinical mastitis.
Bacterial examination of the samples with somatic cell counts of greater than 200,000 cells/mL at QIA and local veterinary clinics was carried out using standard laboratory techniques (National Mastitis Council, 1990) . Escherichia coli was identified using a Vitek MS v2.0 system (bioMérieux, Durham, NC). One isolate per sample was selected for antimicrobial susceptibility tests.
Bacterial examination of the samples with somatic cell counts of greater than 200,000 cells/mL at QIA and local veterinary clinics was carried out using standard laboratory techniques (National Mastitis Council, 1990) . Escherichia coli was identified using a Vitek MS v2.0 system (bioMérieux, Durham, NC). One isolate per sample was selected for antimicrobial susceptibility tests.
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