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2 protocols using rabbit anti pink1

1

PINK1 and BCKDE1A Phosphorylation Analysis

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The PVDF membrane was probed with rabbit anti-PINK1 (#6946P; Cell Signaling), rabbit anti- Phospho (S293) BCKDE1A (#BET A304-672A-M; Bethyl Laboratories), anti-BCKDE1A (H-5) (#sc-271538; Santa Cruz Biotechnology) and mouse β-actin (#A5541; Sigma-Aldrich), followed by goat-anti-mouse or goat-anti-rabbit labeled with IRDye infrared dyes (LI-COR Biosciences, Leusden, Netherlands). A LI-COR Odyssey CLX infrared imaging system (LI-COR) was used for fluorescence detection.
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2

Mitochondrial Dynamics Regulation Assay

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Chemicals were purchased from Sigma-Aldrich (Deisenhofen, Germany) except Mdivi-1 (Enzo Life Science, Lörrach, Germany) as well as doxycycline, blasticidin and zeocin (Invivogen, San Diego, CA, USA). Cell culture materials and media were obtained from Biochrom (Berlin, Germany). MitoTracker GreenFM (MTG) and MitoSOX™ Red superoxide indicator (MitoSOX) were purchased from Molecular Probes (Eugene, USA).
The following primary antibodies were used: rabbit anti-DNP (Sigma-Aldrich, Deisenhofen, Germany), rabbit anti-PINK1 (Cell Signaling, Boston, MA, USA), mouse anti-β-actin (Cell Signaling, Boston, USA), rabbit anti-COX IV (Cell Signaling, Boston, MA, USA), rabbit anti-Lon protease (Abcam, Cambridge, UK), mouse anti-GAPDH (Abcam, Cambridge, UK), rabbit anti-Ki-67 (Abcam, Cambridge, UK), mouse anti-CDKN2A/p16INK4α (Abcam, Cambridge, UK), rabbit anti-p21 Waf1/Cip1 (Cell Signaling, Boston, MA, USA), mouse anti-MT-CO1 (Abcam, Cambridge, UK), mouse anti-SDHA (Abcam, Cambridge, UK), mouse anti-VDAC (Abcam, Cambridge, UK) and rabbit anti-Fis1 (Cell Signaling, Boston, MA, USA). Secondary antibodies used for immunoblotting were purchased from LI-COR Biosciences (Lincoln, AL, USA). The FITC-labeled antibody for immunofluorescence was purchased from Invitrogen (Carlsbad, CA, USA).
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