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0.1n iodine solution

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0.1N iodine solution is a laboratory reagent used for various titration and analysis procedures. It is a standardized aqueous solution containing 0.1 moles of iodine per liter. The solution's core function is to serve as a titrant or indicator in chemical reactions and analyses that require precise iodometric determinations.

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Lab products found in correlation

Bis acrylamide, by Bio-Rad (3 mentions) Saponin, by Merck Group (3 mentions) Acrylamide, by Bio-Rad (2 mentions) Skyscan 1272, by Bruker (1 mentions)

Spelling variants of this product

Iodine (122 citations) Iodine solution (9 citations)

4 protocols using 0.1n iodine solution

1

Hydrogel Stabilization and Iodine Staining of E15.5 Embryos

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Each E15.5 embryo was subjected to hydrogel stabilization2 (link). Briefly, the embryo was incubated in 20 mL hydrogel solution containing a mixture of ice-cold 4% (wt) PFA, 4% (wt/vol) acrylamide (Bio-Rad, Mississauga, ON, Canada), 0.05% (wt/vol) bis-acrylamide (Bio-Rad, Mississauga, ON, Canada), 0.25% VA044 Initiator (Wako Chemicals USA, Inc., Richmond, VA, USA), 0.05% (wt/vol) saponin (Sigma-Aldrich, St Louis, MO, USA) and PBS at 4°C for 3 days. After incubation, the tube containing the embryo was placed in a dessication chamber where air in the tube was replaced with nitrogen gas. The tube was placed in a 37°C water bath for 3 hours. Lastly, the samples were separated from the encasing gel and place into iodine solution. Each E15.5 mouse embryo was stained with 50 mL of 0.1N iodine solution (Sigma-Aldrich) for 24 hours. The iodine-stained embryo was then embedded in agarose within an 11-mm centrifuge tube and positioned in the micro-CT scanner for imaging.
Dickinson M.E., Flenniken A.M., Ji X., Teboul L., Wong M.D., White J.K., Meehan T.F., Weninger W.J., Westerberg H., Adissu H., Baker C.N., Bower L., Brown J.M., Caddle L.B., Chiani F., Clary D., Cleak J., Daly M.J., Denegre J.M., Doe B., Dolan M.E., Edie S.M., Fuchs H., Gailus-Durner V., Galli A., Gambadoro A., Gallegos J., Guo S., Horner N.R., Hsu C.W., Johnson S.J., Kalaga S., Keith L.C., Lanoue L., Lawson T.N., Lek M., Mark M., Marschall S., Mason J., McElwee M.L., Newbigging S., Nutter L.M., Peterson K.A., Ramirez-Solis R., Rowland D.J., Ryder E., Samocha K.E., Seavitt J.R., Selloum M., Szoke-Kovacs Z., Tamura M., Trainor A.G., Tudose I., Wakana S., Warren J., Wendling O., West D.B., Wong L., Yoshiki A., MacArthur D.G., Tocchini-Valentini G.P., Gao X., Flicek P., Bradley A., Skarnes W.C., Justice M.J., Parkinson H.E., Moore M., Wells S., Braun R.E., Svenson K.L., de Angelis M.H., Herault Y., Mohun T., Mallon A.M., Henkelman R.M., Brown S.D., Adams D.J., Lloyd K.C., McKerlie C., Beaudet A.L., Bucan M, & Murray S.A. (2016). High-throughput discovery of novel developmental phenotypes. Nature, 537(7621), 508-514.
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2

Hydrogel Stabilization and Iodine Staining of E15.5 Embryos

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Each E15.5 embryo was subjected to hydrogel stabilization2 (link). Briefly, the embryo was incubated in 20 mL hydrogel solution containing a mixture of ice-cold 4% (wt) PFA, 4% (wt/vol) acrylamide (Bio-Rad, Mississauga, ON, Canada), 0.05% (wt/vol) bis-acrylamide (Bio-Rad, Mississauga, ON, Canada), 0.25% VA044 Initiator (Wako Chemicals USA, Inc., Richmond, VA, USA), 0.05% (wt/vol) saponin (Sigma-Aldrich, St Louis, MO, USA) and PBS at 4°C for 3 days. After incubation, the tube containing the embryo was placed in a dessication chamber where air in the tube was replaced with nitrogen gas. The tube was placed in a 37°C water bath for 3 hours. Lastly, the samples were separated from the encasing gel and place into iodine solution. Each E15.5 mouse embryo was stained with 50 mL of 0.1N iodine solution (Sigma-Aldrich) for 24 hours. The iodine-stained embryo was then embedded in agarose within an 11-mm centrifuge tube and positioned in the micro-CT scanner for imaging.
Dickinson M.E., Flenniken A.M., Ji X., Teboul L., Wong M.D., White J.K., Meehan T.F., Weninger W.J., Westerberg H., Adissu H., Baker C.N., Bower L., Brown J.M., Caddle L.B., Chiani F., Clary D., Cleak J., Daly M.J., Denegre J.M., Doe B., Dolan M.E., Edie S.M., Fuchs H., Gailus-Durner V., Galli A., Gambadoro A., Gallegos J., Guo S., Horner N.R., Hsu C.W., Johnson S.J., Kalaga S., Keith L.C., Lanoue L., Lawson T.N., Lek M., Mark M., Marschall S., Mason J., McElwee M.L., Newbigging S., Nutter L.M., Peterson K.A., Ramirez-Solis R., Rowland D.J., Ryder E., Samocha K.E., Seavitt J.R., Selloum M., Szoke-Kovacs Z., Tamura M., Trainor A.G., Tudose I., Wakana S., Warren J., Wendling O., West D.B., Wong L., Yoshiki A., MacArthur D.G., Tocchini-Valentini G.P., Gao X., Flicek P., Bradley A., Skarnes W.C., Justice M.J., Parkinson H.E., Moore M., Wells S., Braun R.E., Svenson K.L., de Angelis M.H., Herault Y., Mohun T., Mallon A.M., Henkelman R.M., Brown S.D., Adams D.J., Lloyd K.C., McKerlie C., Beaudet A.L., Bucan M, & Murray S.A. (2016). High-throughput discovery of novel developmental phenotypes. Nature, 537(7621), 508-514.
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3

Micro-CT Analysis of Mouse Penises

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Penises were dissected from C57BL6/J adult wild-type mice and fixed overnight in 10% formalin. The fixed tissue was washed twice with 70% ethanol, followed by dehydration and storage in 70% ethanol. 24 hours before micro-CT scanning, iodine staining was achieved by submerging the samples in a 0.1 N iodine solution (Sigma-Aldrich, St Louis, MO, USA) on a shaker at 4°C. Before scanning, the samples were rinsed with 1 × phosphate buffered saline and suspended in a sample tube with 1% agarose.
O’Neill M., Huang G.O, & Lamb D.J. (2017). Novel Application of Micro-Computerized Tomography for Morphologic Characterization of the Murine Penis. The journal of sexual medicine, 14(12), 1533-1539.
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4

Mouse Embryo Micro-CT Imaging

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Embryos were subjected to hydrogel stabilization before imaging.21 Briefly, each embryo was placed in 20 mL hydrogel solution: ice-cold 4% paraformaldehyde (wt), 0.05% (wt/vol) bis-acrylamide (Bio-Rad, Mississauga, ON, Canada), 0.25% VA044 Initiator (Wako Chemicals USA, Inc., Richmond, VA, USA), 0.05% (wt/vol) saponin (Sigma–Aldrich, St Louis, MO, USA) and PBS at 4 °C for 3 days, then placed in a desiccation chamber where air was replaced with nitrogen gas, followed by placement in a 37 °C water bath for 3 h. The sample was then separated from the encasing gel and placed into 50 mL of 0.1 N iodine solution (Sigma–Aldrich) for 24 h, washed in PBS for 1 h, and embedded in 1% agarose prior to imaging.
Three-dimensional data sets were acquired for mouse embryos using a Bruker Skyscan 1272 micro-CT scanner (Bruker Skyscan, Antwerp, Belgium), with the X-ray source at 100 kV and 100 μA. The specimen was rotated 360° in 0.3° increments, generating 1200 views that were reconstructed into data blocks with a 11-μm voxel size. The 3D video models for the embryos were created using the Amira Software.
Mohan H., Nguyen J., MacKenzie B., Yee A., Laurette E.Y., Sanghvi T., Tejada O., Dontsova V., Leung K.Y., Goddard C., De Young T., Sled J.G., Greene N.D., Copp A.J, & Serghides L. (2023). Folate deficiency increases the incidence of dolutegravir-associated foetal defects in a mouse pregnancy model. eBioMedicine, 95, 104762.
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