The sections were first dipped in a prewarmed Oil Red O solution (0.18% in 60% isopropanol) for 1 h at 60°C, then rinsed twice with water for 5 min each. The slides were then dipped in hematoxylin solution for 5 min, rinsed, and mounted in 20% glycerol. Images were acquired using Leedz Microimaging 5 MP camera attached to a Nikon Ti-U microscope. Analysis was performed by four individuals independently including a trained pathologist.
Microimaging 5 mp camera
Manufactured by Nikon
The Microimaging 5 MP camera is a high-resolution digital camera designed for laboratory and microscopy applications. It features a 5-megapixel sensor that captures detailed images for various scientific and research purposes. The camera provides a straightforward and reliable solution for microscopic imaging and analysis tasks.
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Lab products found in correlation
2 protocols using microimaging 5 mp camera
1
Lipid Staining and Microscopic Analysis
2
Lipid Staining and Quantification in Lung Tissue
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The sections were first dipped in a pre-warmed Oil Red O (Cat no. 09755, Sigma) solution (0.18% in 60% isopropanol) for 1 h at 60°C, and then rinsed twice with water for 5 min each. The slides were then dipped in hematoxylin solution for 5 min, rinsed, and mounted in 90% glycerol. Images were acquired using Leedz Microimaging 5 MP camera attached to a Nikon Ti-U microscope. For quantifications of the signal, whole lung sections were scanned using a 1800dpi document scanner and red channel intensity quantified using Fiji. The Lab color space module was chosen to select thresholds.
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