Osilodrostat

Manufactured by Selleck Chemicals

Osilodrostat is a laboratory equipment product manufactured by Selleck Chemicals. It is a selective inhibitor of the enzyme 11β-hydroxylase, which is involved in the biosynthesis of cortisol.

Automatically generated - may contain errors

Lab products found in correlation

Mitotane, by Selleck Chemicals (1 mentions) Ketoconazole, by Selleck Chemicals (1 mentions) Roscovitine, by Selleck Chemicals (1 mentions) Gant61, by STEMCELL (1 mentions) Mts assay, by Promega (1 mentions) Fadrozole, by Merck Group (1 mentions) Progesterone, by Merck Group (1 mentions) 11 deoxycorticosterone, by Merck Group (1 mentions) Corticosterone, by Merck Group (1 mentions)

3 protocols using osilodrostat

Pituitary Organoid Cytotoxicity Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols

Organoids were grown in Matrigel™ domes within 96-well round-bottom culture plates. Recombinant human SHH was removed from the pituitary organoid growth media, 24 h prior to drug treatment. Organoids were treated with either vehicle (DMSO), cabergoline (Selleckchem S5842), ketoconazole (Selleckchem S1353), roscovitine (Selleckchem S1153), GANT61 (Stemcell Technologies 73692), pasireotide (TargetMol TP2207), mifeprostone (Selleckchem S2606), etomidate (Selleckchem S1329), mitotane (Selleckchem S1732), metyropane (Selleckchem S5416), or osilodrostat (Selleckchem S7456) at concentrations of 0, 1, 10, 100, 1000, and 10,000 nM, for 72 h. The percentage of cell viability was measured using an MTS assay (Promega G3580). Absorbance was measured at 490 nm and normalized to the vehicle. Concentrations were plotted in a logarithmic scale, and a nonlinear dose response curve regression was calculated using GraphPad Prism. An IC50 value for each drug treatment was determined based on the dose response curve, using GraphPad Prism analysis software.

Chakrabarti J., Pandey R., Churko J.M., Eschbacher J., Mallick S., Chen Y., Hermes B., Mallick P., Stansfield B.N., Pond K.W., Thorne C.A., Yuen K.C., Little A.S, & Zavros Y. (2022). Development of Human Pituitary Neuroendocrine Tumor Organoids to Facilitate Effective Targeted Treatments of Cushing’s Disease. Cells, 11(21), 3344.

+ Open protocol

+ Expand

Osilodrostat Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

LCI699 (osilodrostat or Isturisa) (CAS 928134-65-0) was purchased from Selleckchem. Progesterone (CAS 57-83-0), corticosterone (CAS 50-22-6), 11-deoxycorticosterone (CAS 64-85-7), and fadrozole (CAS 102676-31-3) were purchased from Sigma-Aldrich.

Brixius-Anderko S, & Scott E.E. (2021). Structural and functional insights into aldosterone synthase interaction with its redox partner protein adrenodoxin. The Journal of Biological Chemistry, 296, 100794.

+ Open protocol

+ Expand

Osilodrostat and Steroidal Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

LCI699 (osilodrostat or Isturisa^®) (CAS 928134-65-0) was purchased from Selleckchem. Progesterone (CAS 57-83-0), cortisol (50-23-7), and 11-deoxycortisol (152-58-9) were obtained from Sigma-Aldrich (St. Louis, MO). (R)- and (S)-Fadrozole were a gift from Pfizer.

Loomis C.L., Brixius-Anderko S, & Scott E.E. (2022). Redox Partner Adrenodoxin Alters Cytochrome P450 11B1 Ligand Binding and Inhibition. Journal of inorganic biochemistry, 235, 111934.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!