Fa free bovine serum albumin

Collagenase P and FA-free Bovine serum albumin were obtained from Roche Diagnostics S.L. (Barcelona, Spain). Bovine serum albumin and most of the substances, inhibitors (POM–1, NPPB, carbenoxolone, flufenamic acid, mefloquine, diazoxide), activators (bzATP), enzymes and coenzymes were obtained from Sigma-Aldrich Química S.A. (Madrid, Spain). Other inhibitors used (ARL–67156, CGS15943, suramin) were from Tocris Bioscience (Biogen Científica S.L., Spain). Rat insulin standards were from Linco Research, Inc. (St. Charles, Missouri, U.S.A.). Na¹²⁵I was obtained from PerkinElmer España, S.L. (Madrid, Spain). Ad-CMV-Luciferase was from Vector Biolabs (Philadelphia, PA, U.S.A.). Inorganic compounds and organic solvents were obtained from VWR International Eurolab S.L. (Spain).

Collagenase P and FA-free bovine serum albumin were obtained from Roche Diagnostics S.L. (Barcelona, Spain). Bovine serum albumin and most of the substances (metabolites, cofactors, inhibitors) were obtained from Sigma-Aldrich Química S.A. (Madrid, Spain). Rat insulin standards were from Linco Research, Inc. (St. Charles, Missouri, U.S.A.). Na¹²⁵I was obtained from PerkinElmer España, S.L. (Madrid, Spain). Inorganic compounds and organic solvents were obtained from VWR International Eurolab S.L. (Spain).