Fluorescence-activated cell-sorting analysis for p53 was performed. PE-labeled anti-human p53 antibody (Mouse monoclonal, BD Biosciences) was used. For dead cell exclusion, 20 μl of 7-AAD (BD pharmingenTM) was added before analysis. Fluorescence intensity was analyzed using the BD FACS Calibor Flow cytometer (BD Biosciences) and the data were analyzed using CELL Quest software (Ver. 2.0.1) (BD Biosciences).
Facs calibor flow cytometer
Manufactured by BD
The BD FACS Calibor is a flow cytometer designed for the analysis of cells and particles. It utilizes laser-based technology to detect and measure various physical and chemical characteristics of cells or particles as they flow through a fluid stream. The core function of the BD FACS Calibor is to provide quantitative data on the properties of individual cells or particles within a heterogeneous sample.
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Lab products found in correlation
2 protocols using facs calibor flow cytometer
1
p53 Expression Analysis by FACS
2
MRGPRX4 Receptor Expression Analysis
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Suspended live HEK 293 cells stably expressing the point-mutated MRGPRX4 were washed in washing buffer (1X PBS solution, mixed with 5% fatal bovine serum [FBS]) for 3 times. Then cells were incubated with the rabbit anti-c-Myc primary antibody (Sigma-Aldrich cat. # C3956, 1:25 dilution) for 30 min, and secondary antibody (AAT Bioquest iFluro Alexa 488 goat antirabbit IgG, cat. # 1060423, 1:50 dilution) for 1 hr. Cells were washed for two times after each antibody treatment. Next, cells were resuspended with 300 uL to 500 uL FACS buffer, and fluorescence-activated cell-sorting analysis was performed, using the BD FACS Calibor Flow cytometer (BD Biosciences), and the data were analyzed using FlowJo software (Ver. 7.6.1).
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