IHC for target molecules was performed on serial sections from tumor tissues of nude mice xenografts and CRC patients. Tissue sections were deparaffinized, subjected to antigen retrieval using target antigen retrieval solution (Dako), and incubated with primary antibodies against Ki67 (Dako M7240), cleaved Caspase-3 (CST #9661), β-catenin (BD Biosciences #610154), GATA6 (Abcam #22600), DKK1 (Abcam #61034), DKK3 (Abcam #115869), ZNRF3 (Abcam #122353), RNF43 (Abcam #129401), and APC2 (Abcam #113370). Then sections were incubated with Envision System HRP-labeled polymer anti-rabbit or anti-mouse secondary antibodies (Dako). The results of IHC were scored by two independent observers. Ki-67 and cleaved Caspase-3 staining was quantified by calculating positively stained cells in at least 5 randomly chosen HPFs of each sample. Quantification of other molecules was based on intensity and extent of staining according to the histological scoring method as previously described68 (link).
Envision system hrp labeled polymer anti rabbit or anti mouse secondary antibodies
Manufactured by Agilent Technologies
Envision System HRP-labeled polymer anti-rabbit or anti-mouse secondary antibodies are laboratory reagents designed to detect and visualize target proteins in immunohistochemical and immunocytochemical applications. These polymer-based secondary antibodies are conjugated with horseradish peroxidase (HRP) enzyme, which can catalyze a color-producing reaction for signal amplification and detection.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using envision system hrp labeled polymer anti rabbit or anti mouse secondary antibodies
1
Immunohistochemical Profiling of Xenograft and CRC Tissues
2
Immunohistochemical Profiling of Xenograft and CRC Tissues
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!