Synthesized MTHP was dissolved in 0.1 L water, then 65% water solution of DADMAC was added to a combined volume of 0.2 L, comonomer ratio 1:1, combined comonomer concentration either 1 or 2 mol/L. 0.228 g PSA was added under constant agitation, then polymerization was carried out at 60 °C for 20 h, followed by sedimentation into acetone. Polymer was additionally purified from monomers by dialysis against distilled water. Copolymer yield was 40 to 51%.
Elemental composition was determined via pyrolysis chromatography on a Thermo Flash 2000 Organic Elemental Analyzer (Thermo Fisher Scientific, Waltham, MA, USA), sample mass 1–4 mg pyrolyzed at 2000 °C in helium carrier gas, to a precision of 3–5%.
Intrinsic viscosity measurements were performed in an Ubbelohde type viscometer (Fungilab, Barcelona, Spain) in water and 0.5 N NaCl solution in water at 30 °C.
Monomer conversion was determined gravimetrically, determined via weighing of the precipitated polymer.
1H and 13C NMR spectra were collected on a Bruker (Billerica, MA, USA) spectrometers DRX500 (500.13 MHZ for 1H and 125.76 MHz for 13C), and MDS-300 (300 MHz) in D2O and DMSO-d6 at 20 °C, chemical shifts relative to solvent residual protons.
Elemental composition was determined via pyrolysis chromatography on a Thermo Flash 2000 Organic Elemental Analyzer (Thermo Fisher Scientific, Waltham, MA, USA), sample mass 1–4 mg pyrolyzed at 2000 °C in helium carrier gas, to a precision of 3–5%.
Intrinsic viscosity measurements were performed in an Ubbelohde type viscometer (Fungilab, Barcelona, Spain) in water and 0.5 N NaCl solution in water at 30 °C.
Monomer conversion was determined gravimetrically, determined via weighing of the precipitated polymer.
1H and 13C NMR spectra were collected on a Bruker (Billerica, MA, USA) spectrometers DRX500 (500.13 MHZ for 1H and 125.76 MHz for 13C), and MDS-300 (300 MHz) in D2O and DMSO-d6 at 20 °C, chemical shifts relative to solvent residual protons.